中文摘要：

目的：观察成牙本质细胞中是否表达成纤维细胞生长因子18。 方法：实验于2005—02／04在解放军第四军医大学口腔医院口腔内科实验室完成。①小鼠成牙本质样细胞系MDPC-23培养：细胞培养在含体积分数为0．1的胎牛血清α—MEM、青霉素100IU／mL、链霉素100mg／L和谷氨酰胺50mg／L的完全培养液中，同时附加50mg／L抗坏血酸。②免疫组织化学染色：一抗用1：100稀释的成纤维细胞生长因子18多克隆抗体，4℃湿盒过夜，最后滴加二氨基联苯胺液显色。以磷酸盐缓冲液代替一抗设为阴性对照。成纤维细胞生长因子18阳性表达细胞胞浆呈棕黄色。③免疫荧光染色：一抗用1：50稀释的羊抗小鼠成纤维细胞生长因子18多克隆抗体，4℃湿盒过夜后滴加按1：500稀释的FITC标记的兔抗羊二抗。阴性对照用磷酸盐缓冲液代替一抗进行染色。成纤维细胞生长因子18阳性表达细胞在荧光显微镜下发出绿色荧光。 结果：免疫荧光和免疫组织化学法均表明成纤维细胞生长因子18在MDPC-23细胞浆呈阳性表达。 结论：从蛋白水平证实了成牙本质样细胞MDPC-23表达成纤维细胞生长因子18，提示成纤维细胞生长因子18可能是影响成牙本质细胞分化的胞内信号转导分子。

英文摘要：

AIM： To investigate the expression of fibroblast growth factor 18 in odontoblast cell line. METHODS： The experiment was carried out in laboratory of stomatology, Hospital of Stomatology of the Fourth Military Medical University of Chinese PLA from February to April 2005. ①Cell line MDPC-23 culture： Odontoblast cell line MDPC-23 was cultured in α-MEM containing 0.1 volume fraction fetal bovine serum （V/V）, 100 IU/mL benzylpenicillin, 100 mg/mL streptomycin, 50 mg/mL glutamine and 50 mg/mL ascorbic acid.②Immunohistochemical staining： 1：100 diluted fibroblast growth factor 18 muhiclonal antibody was used as primary antibody and phosphate buffer as control. Then the samples were incubated in moist-chamber overnight at 4 ℃. After reaction with diaminobenzidine, fibroblast growth factor 18 positive cells were brown colored. ③Immunofluorescent staining： 1：50 diluted goat anti mouse fibroblast growth factor 18 muhiclonal antibody was used as primary antibody and phosphate buffer as control. Then the samples were incubated in moist-chamber overnight at 4 ℃ and 1：500 diluted rabbit anti goat monoclonal antibody labeled with FITC was used as secondary antibody, fibroblast growth factor 18 positive cells were green colored under fluorescence microscope. RESULTS： Fibroblast growth factor 18 was expressed in cytoplasm of MDPC-23 cell by both immunohistochemical and immunofluorescent stainings. CONCLUSION： The expression of fibroblast growth factor 18 at protein level in MDPC-23 is proved,which indicated that fibroblast growth factor 18 may be a signal factor that participates in the differentiation of odontoblast.