中文摘要：

目的 制备特异性的甘草酸单克隆抗体。方法 通过活性酯法分别制备了甘草酸免疫抗原GA-KLH和包被抗原GA-OVA;用免疫抗原免疫Balb/c小鼠,采用间接竞争ELISA法筛选后,成功获得了能稳定分泌甘草酸单克隆抗体的杂交细胞株3D6;用阳性细胞株刺激小鼠,获得了高效价的、特异性很高的甘草酸单克隆抗体。结果 经检测与甘草酸主要结构类似物甘草次酸的交叉反应率低于0.5%,与熊果酸、齐墩果酸等甘草酸结构类似物无交叉反应;将该方法应用于甘草样品实测,采用HPCL法验证,结果表明：所采用的ELISA分析方法测定的数据与HPLC分析方法有很好的相关性（r^2=0.9977）。结论为甘草酸ELISA快速检测试剂盒的开发奠定了良好的基础。

英文摘要：

OBJECTIVE To prepare a specific monoclonal antibody against glycyrrhizic acid（GA）. METHODS hnmunizing antigen GA - KLH and envelope antigen GA - OVA were respectively prepared employing active ester method. Balb/c mice were made immune through immunizing antigen. After screening through ELISA, an indirect competition method,3D6, a hybrid cell line that was able to stably secrete monoclonal antibody of GA was successfully obtained. GA monoclonal antibody with high potency and very high specificity was obtained through stimulaling mice with positive cells. RESULTS According to the test, the rate of cross reaction with GA which was similar with the main structure of GA was lower than 0.5% and there was no cross reaction with articles owning similar structure as GA, such as ursolic acid and oleanolic acid. The contents GA determined by ELISA agreed well with those determined by HPLC. The ELISA results were good correlation with HPLC ; the correlation was r^2 = 0. 9977. CONCLUSION The method has laid a good foundation for the development of GA ELISA rapid analysis.