在营养液培养条件下,对两个不同氮效率基因型水稻品种扬稻6号和武育粳3号采用光呼吸抑制剂异烟肼(INH)和谷氨酰胺合成酶(GS)抑制剂蛋氨酸亚砜亚胺(MSO)处理,研究其对水稻光合速率、光呼吸速率、GS酶活性及冠层的NH。挥发速率的影响。结果发现:(1)MSO导致剑叶光合速率下降,光呼吸速率升高;INH导致光呼吸速率显著下降,同时一定程度上引起光合速率降低。(2)MSO处理显著降低了GS酶活性,相应地引起NH。挥发速率增加;INH在一定程度上导致NH。挥发速率降低。(3)扬稻6号NH。挥发速率比武育粳3号低的生理原因是光呼吸速率较低和GS酶活性较高。
Pot experiments were conducted using Yoshida culture solution with two rice varieties (Wuyujing-3 and Yangdao-6) to study the effects of glutamine synthetase inhibitor methionine sulfoximine (MSO) and photorespiration inhibitor isonicotinyl hydrazine (INH) on the ammonia volatilization rate (AVR), photorespiration rate(Pr) and glutamine synthetase (GS) activity in rice. The results showed that: ( 1 ) MSO decreased the Pn, but increased the Pr in the flag leaf;INH decreased the Pr significantly and Pn slightly. (2)MSO decreased GS activity, which promoted an increase in AVR in rice canopy. INH showed a slightly positive effect on the decrease in AVR. (3) Based on the comparison between the two tested varieties, the higher AVR variety (Yangdao-6) showed greater GS activity and lower Pr than those of the lower AVR variety (Wuyuiinq-3).