筛选出一株能分泌胞外酶、促进污泥溶解的嗜热脂肪芽孢杆菌Bacillus stearothermophilus sp,AT06-2,将AT06-2培养液接种人不同浓度(TSS约为7.14和21 g·L^-1)的剩余污泥,于60℃进行嗜热菌溶解试验,并与未接种试验进行对比,结果表明,接种后TSS和VSS溶解率都得到提高,60h后接种试验VSS溶解率分别达到58.76%、50.17%和39.38%,而未接种试验VSS溶解率仅为34.00%、28.63%和25.22%,在固形物质溶解机理方面,VSS的溶解主要受酶催化效应和热水解效应的影响,TSS的溶解减量取决于VSS和FSS的组成比例,VSS溶解在初始阶段(t=3d)符合准一级反应动力学模型,接种后溶解速率常数K_d显著提高,分别从未接种的0.14d^-1、0.10d^-1、0.10d^-1相应提高到接种条件下的0.30d^-1、0.23d^-1和0.16d^-1。
A thermophilic bacterium AT06-2 identified as Bacillus stearothermophilus sp. was isolated and shown to produce extraeellular enzymes accelerating to solubilize excess sludge. Sludge solubilization with and without inoculation of AT06-2 was investigated at 60℃ under different total suspended solid (TSS) concentrations (7, 14 and 21 g·L^-1). The results showed that the solubilization of TSS and volatile suspended solids (VSS) were enhanced by inoculation with the bacteria. The VSS solubilization rate reached 58.76% , 50. 17% and 39.38% 60 hours after the inoculation, compared to 34.00% , 28.63% and 25.22% of VSS solubilized without inoculation under the same conditions. VSS solubilization was mainly affected by enzyme-catalysis and thermal hydrolysis, and TSS minimization was largely determined by the component ratio of VSS and fixed suspended solid (FSS). Pseudo-first order reaction kinetics could adequately describe VSS solubilization for the first 3 days and the soluhilization rate constant K_d (t=3d) inereased greatly from0.14 d^-1, 0.10d^-1 and0.10 d^-1 to 0.30 d^-1, 0.23d^-1and0.16d^-1 with AT06-2 inoculation.