中文摘要：

目的探索天龙粉经过4种蛋白酶酶解后对肺腺癌A549细胞株的抑制作用,筛选出最佳用酶。方法天龙粉分别经过胰蛋白酶、胃蛋白酶、胰凝乳蛋白酶和弹性蛋白酶酶解,采用茚三酮比色法测定蛋白质的水解度,同时以天龙粉水提物作为对照组,用甲基噻唑蓝（MTT）法测定4种酶水解物对肺腺癌A549的抑制率。结果对照组不同药物浓度对肺腺癌A549细胞无明显抑制作用;弹性蛋白酶、胰蛋白酶对天龙粉的水解度分别为77.50%、28.02%,给药组不同药物浓度对肺腺癌A549细胞的抑制率明显高于对照组;胰凝乳蛋白酶和胃蛋白酶的水解度分别为34.63%、72.80%,给药组不同药物浓度对肺腺癌A549细胞无明显抑制作用。结论天龙粉酶解工艺的最佳用酶是弹性蛋白酶,其次是胰蛋白酶。

英文摘要：

Objective To explore the inhibitory effect of Gekko powder after enzymolysis with 4 proteases on lung adenocarcinoma A549 cell line and to select the best enzyme. Methods The Gekko powder was hydrolyzed by trypsin, pepsin, chymotrypsin and elastase, respectively. Indene three ketone colorimetric method was used to measure the hydrolysis degree of protrin. Meanwhile methyl thiazolyl blue（MTT） method was used to measure inhibition rate of the enzymatic hydrolysates on lung adenocarcinoma A549 cell line, with water extracts of the Gekko powder as the control group. Results The control group had no obvious inhibitory effect on lung adenocarcinoma A549 cell line at different concentrations of drugs. The hydrolysis degree of elastase and trypsin was 77.50%, and 28.02%, and the inhibition of A549 cell line at different drug concentrations was significantly higher than that of the control group. The hydrolysis degree of chymotrypsin and pepsin was 34.63% and 72.80% respectively, without significant inhibition. Conclusion Elastase is the best in enzymolysis technology for Gekko powder, followed by trypsin.