中文摘要：

目的 观察姜黄素对饮水型砷中毒大鼠肝脏氧化损伤的干预作用.方法 选取SD大鼠32只,采用随机数字表法按体质量分为4组,每组8只,雌雄各半.正常对照组给予去离子水灌胃135 d;砷中毒对照组按体质量给予10 mg/kg含砷溶液灌胃90d后,再用去离子水灌胃45 d;单纯姜黄素组按体质量给予1 000mg/kg姜黄素溶液灌胃135 d;姜黄素治疗组按体质量给予10 mg/kg含砷溶液灌胃90 d后,再用1 000 mg/kg姜黄素溶液灌胃治疗45 d.采用氢化物发生-电感耦合等离子体发射光谱法（HG-ICP-OES）测定4组大鼠尿砷、肝砷含量;比色法检测血清及肝脏氧化损伤相关指标[铜锌超氧化物歧化酶（Cu/Zn-SOD,简称SOD1）、过氧化氢酶（CAT）活力和丙二醛（MDA）含量];免疫印迹法检测肝脏抗氧化酶SOD1、CAT蛋白表达.结果 与正常对照组大鼠尿砷和肝砷[（0.40±0.14）μg/g Cr,（4.56±1.05）μg/g]含量比较,砷中毒对照组大鼠尿砷[（5.83±0.29）μg/g Cr]和肝砷[（15.76±1.65）μg/g]含量明显升高（P均＜0.05）,姜黄素治疗组尿砷[（1.07±0.14）μg/gCr]含量明显升高（P＜0.05）;与砷中毒对照组相比,姜黄素治疗组尿砷和肝砷[（5.42±1.76）μg/g]明显下降（P均＜ 0.05）.与正常对照组大鼠血清和肝脏SOD1、CAT、MDA含量[（102.46±5.03）、（29.33±8.13）U/ml,（3.11±0.49） μmol/L和（204.05±18.33）、（126.26±13.19）U/mg prot,（1.62±0.42）μmol/g prot]比较,砷中毒对照组和姜黄素治疗组血清和肝脏的SOD1、CAT活力[（60.97±7.94）、（13.56±5.14）U/ml,（133.66±1151）、（74.01±13.30）U/mg prot;（87.39±9.38）、（20.45±6.49）U/ml,（178.27±9.32）、（93.70±20.35）U/mg prot]明显降低,MDA含量[（7.26±0.54）μmol/L,（2.61±0.52）μmol/g prot;（4.34±0.79） μmol/L,（1.92±0.18） μmol/gprot]明显升高（P均＜0.05）;与砷中毒对照组相比,姜黄素治疗组血清和肝脏的SOD1、CAT活力升高,MDA含量降低（P均＜ 0.05）.

英文摘要：

Objective To observe the effects of curcumin on hepatic oxidant stress in water arsenic-exposed rats and to study its mechanism,which can offer references for curcumin used in antioxidant therapy of arsenic poisoning.Methods Thirty-two SD rats were divided into 4 groups according to body weight by random number table,half male and half female.Including control group （lavaged 135 days with deionized water）,arsenic poisoning group （lavaged 45 days with deionized water after lavaging 90 days with 10 mg/kg sodium arsenite）,pure curcumin group （lavaged 135 days with 1 000 mg/kg curcumin solution） and curcumin treatment group （lavaged 45 days with 1 000 mg/kg curcumin solution after lavaging 90 days with 10 mg/kg sodium arsenite）,8 rats in each group.The arsenic contents of urine （urine creatinine corrected） and liver were detected by hydride generation inductively coupled plasma optical emission spectrometer （HG-ICP-OES）;the activity of Cu/Zn-superoxide dismutase （SOD1） and catalase （CAT）,the contents of malondialdehyde （MDA） in serum and liver homogenate by colorimetric method;the protein expression of liver antioxidant enzyme （SOD 1 and CAT） was assayed by Western blotting.Results The arsenic contents of urine and liver in arsenic poisoning group [（5.83 ± 0.29）μg/g Cr,（15.76 ± 1.65）μg/g] and the arsenic contents of urine in curcumin treatment group [（1.07 ± 0.14）μg/g Cr] were obviously higher than those of control group [（0.40 ± 0.14）μg/g Cr,（4.56 ± 1.05）μg/g,all P 〈 0.05];compared to arsenic poisoning group,the arsenic contents of urine and liver in curcumin treatment group [（1.07 ± 0.14）μg/g Cr,（5.42 ± 1.76）μg/g] were obviously lower （all P 〈 0.05）.The contents of serum and liver SOD1,CAT and MDA in control group respectively were （102.46 ± 5.03）,（29.33 ± 8.13）U/ml,（3.11 ± 0.49）μ mol/L and （204.05 ± 18.33）,（126.26 ± 13.19）U/mg prot,（1.62 ± 0.42） μmol/g prot.Compared to the control,the activity