中文摘要：

建立以哺乳动物α3β4乙酰胆碱受体（nAChR）为分子靶标的药物筛选模型。将大鼠乙酰胆碱受体的α3与β4亚基基因分别进行体外转录，获得α3与β4亚基基因的cRNA，按1：1的比例将适量α3与β4的cRNA混合后，显微注射到新鲜分离的非洲爪蟾卵母细胞中，在ND96溶液中、17℃下培养24h后，通过双电极电压钳技术（TEVC）检测受体α3β4阱nAChR的乙酰胆碱（Ach）门控电流。同时注入α3、β4cRNA的非洲爪蟾卵母细胞孵育24h后，可记录到明显的Ach门控内向电流，未注射或只注射ddH2O的对照组细胞则没有任何电流产生。α3β4乙酰胆碱受体在非洲爪蟾卯母细胞膜上成功表达，以此为药物作用靶点，可用于大量生物毒素等物质的活性筛选实验模型。

英文摘要：

To establish a drug screening model with the molecular target of mammal α3β4 acetylcholine receptor（nAChR） ,rat α3 and β4 nAChR subunits＇ cRNA were obtained by transcription in vitro, which were mixed with equal amount and then mieroinjected in the fresh oocytes of Xenopus laevis. The oocytes were incubated in ND96 buffer at 17 ℃ over 24 hours after microinjection. Twomicroelectrode voltage clamp（ TEVC ） was used to detect the acetylcholine （Ach） gated current of α3β4 nAChR. The Ach gated inward current was recorded in the α3 and β4 cRNA injected oocytes, while no current was recorded in both blank oocytes and ddH20 injected oocytes. The results demonstrated that the α3β4 nAChR was expressed in Xenopus laevis oocytes successfully. This can be used as the experimental model to screen bioactive toxins with the drug target of α3β4 nAChR.