中文摘要：

目的 观察人饵或牛饵诱捕的野外中华按蚊经实验室传代后嗜血行为的变化。 方法 在山东省单县水稻种植区分别利用人饵和牛饵诱捕大量未吸血的中华按蚊雌蚊，带回实验室分别标记为人饵组和牛饵组，用小白鼠血常规饲养传代；在一大型温室内应用标记-释放-重捕法对两组蚊虫的亲代、子1代、子25代的嗜血性分别进行实验观察。 结果 3次检测的重捕率分别为39.02%（1 332/3 414）、37.97%（2 583/6 803）和30.55%（1 523/4 986）。中华按蚊野外种群人饵组和牛饵组亲代仍在人饵帐和牛饵帐被捕获的比例分别为54.07%（339/627）和58.01%（409/705）（χ^219.42，P﹤0.01）；子1代成蚊两组比例分别为51.03%（669/1 311）和55.11%（701/1 272）（χ^29.75，P﹤0.01）；子25代成蚊两组比例分别为51.98%（342/658）和52.37%（453/865）（χ^22.82，P﹥0.05）。 结论 中华按蚊偏嗜人血或牛血的行为经实验室传25代后可能发生改变。

英文摘要：

Objective To observe the host preference of Anopheles sinensis captured by outdoor human or cattle baits. Methods A large number of non-blood-fed An. sinensis females were collected by overnight trapping outdoor with human and cattle in the rice paddy field in Shan County of Shandong Province, and took back to the lab, and individually labeled as human baits group and cattle baits group, fed with mouse blood. The host preference of parent, F1 and F25 generations of the two groups were observed by mark-release-recapture methods in a large greenhouse. Results The recapture rates of parent, F1 and F25 generations were 39.02% （1 332/3 414）, 37.97% （2 583/6 803）, and 30.55% （1 523/4 986）, respectively. In parent generation, the proportion of mosquitoes from human baits group and cattle baits group collected by human-bait and cattle-bait was 54.07% （339/627） and 58.01% （409/705）, respectively （χ^219.42,P﹤0.01）; in F1 generation, that of the two groups was 51.03% （669/1 311） and 55.11% （701/1 272）, respectively （χ^29.75,P﹤0.01）; in F25 generation, that of the two groups was 51.98% （342/658） and 52.37 （453/865）, respectively （χ^22.82,P﹥0.05）. Conclusion After culture for 25 generations in an experimental condition, the host preference for human or cattle of An. sinensis maybe change.