中文摘要：

目的观察过氧化物酶Ⅰ（PrxⅠ）在正常和骨关节炎（OA）膝关节软骨组织中的表达和分布特点,探讨PrxⅠ与活性氧歧化物和凋亡等在OA软骨表层聚集现象之间的关联。方法分别从正常膝关节提取正常软骨（NC,n=21）和接受膝关节表面置换术的OA患者提取OA软骨（OA,n=21）,应用Westernblot技术检测PrxⅠ在正常软骨细胞和OA软骨细胞中表达水平的总体差异,并用免疫组织化学技术观察PrxⅠ蛋白在正常与OA软骨表/中/深各层组织表达和分布的特点。结果 Western blot证实PrxⅠ在OA软骨组织中的表达水平较正常软骨组织显著提高2.89倍（t=18.34,P〈0.01）。免疫组织化学显示PrxⅠ在正常软骨组织的表层、中层和深层呈现较均一的表达,但是,在OA软骨组织中,PrxⅠ的表达水平存在显著层间差异。PrxⅠ在软骨组织深层表达水平显著升高,但在浅层细胞中,PrxI的表达水平反而显著减低甚至缺如。结论虽然总体表达水平升高,但PrxⅠ在OA软骨组织表层的表达缺如,可能与OA软骨组织表层活性氧歧化物蓄积和细胞凋亡聚集现象相关。

英文摘要：

Objective To investigate the differential expression and distribution of peroxiredoxinⅠ（Prx I） in normal and osteoarthritic knee cartilage,thus to understand the possible correlation between PrxⅠand the superficial accumulating phenomena of reactive oxygen species（ROS） and chondrocyte apoptosis in osteoarthritis（OA） cartilage.Methods Normal cartilage（NC） was obtained from the normal donors（n=21） and osteoarthritic cartilage was harvested from patients who had undergone total knee arthroplasty（n=21）.The overall expression difference of PrxⅠwas compared using Western blot;zonal expression of Prx I inside the normal and osteoarthritic cartilage was detected by immunohistochemistry.Results Overall expression of PrxⅠin osteoarthritic chondrocytes was up-regulated 2.89 fold compared with that of normal cartilage（t=18.34,P0.01）.Immunohistochemistry showed that the expression of PrxⅠwas uniformly in the superficial,middle and deep layes of normal cartilage.But in OA cartilage,the expression of PrxⅠshowed significant differences in the three layers.PrxⅠexpression was up-regulated dominantly in chondrocytes of the deep layer,while the expression was slight,even absent in the superficial layer.Conclusions Although the overall expression of Prx I was up regulated,its expression was down-regulated dramatically in the superficial layer,which might be aetiologically correlated with the accumulation of ROS and chondrocyte apoptosis in the superficial layer of osteoarthritic cartilage.