欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
采用PCR法克隆了桃褐腐病菌的角质酶基因cut1,构建了诱导型表达载体pET28a-cut1,转化大肠杆菌E.coli（BL21）,获得重组菌pET28a-cut1/BL21。经异丙基-β-D-硫代吡喃半乳糖苷（IPTG）诱导,该重组菌角质酶的表达量约为对照菌的1.69倍,酶活约为对照菌的1.8倍,粗酶液的酶活可达40.33 U/mL。
英文摘要：
The cutinase gene cutl has been cloned by a polymerase chain reaction （PCR） from Monolinia fructicola. After ligation to vector pET28a and transformation into E. coli （BL21） , a recombinant strain pET28a-cut1/ BL21 was acquired. Upon isopropyhhio-β-galactoside （IPTG） induction, this recombinant strain produced 1.69 times as much cutinase as the control strain. The maximum cutinase activity of the fermentation broth was 40. 33 U/ mL, nearly 1.8 times that of the control strain.
同期刊论文项目
同项目期刊论文
Enhanced Aldehyde Dehydrogenase Activity by Regenerating NAD+ in Klebsiella pneumoniae and Implicati
期刊信息
位置：