中文摘要：

应用PCR扩增克隆到玫瑰黄链霉菌（Streptomyces mseoflavus）的几丁质酶基因chiC的整个催化域部分，将其与包含信号肽区、纤维素结合区、几丁质结合区三个结构功能域的链霉菌（S．lividans）chiC基因片段相连接，插入pET23b（＋）质粒上，构建表达载体pLCH，用来转化大肠杆菌（Escherichia coli）JM109（DE3），转化子进行破壁之后在胶体几丁质酶培养基上表现活性。

英文摘要：

After getting the chiC catalytic domain of Streptomyces roseoflavus by PCR, the catalytic domain was connected with S. lividans chiC fragment which contains three domains （cellulose binding domain, chitin-binding domain and signal peptide domain） and cloned into pET23b（＋）. Then the recombinant plasmid pLCH was transformed into Eschcrichia coil JM109（DE3）.After the transformat expression was induced with IPTG, its chitinasc activity was found on the chitin culture medium.