中文摘要：

以大雪兰种子为试材,进行无菌播种试验,找出了最适合大雪兰种子萌发、原球茎增殖分化及生根的培养基。结果表明：利于大雪兰种子萌发的培养基是1/2MS＋6-BA 0.3mg/L＋NAA 0.2mg/L＋椰子汁（CM）50mL/L;利于大雪兰原球茎增殖分化的培养基是1/2 MS＋6-BA 0.4mg/L＋NAA 0.2mg/L＋AC 0.5g/L;利于生根壮苗的培养基是1/2 MS＋NAA 0.5mg/L＋AC 2g/L。

英文摘要：

The Cymbidiummastersii Griff,seeds were used to sow in the aseptic conditions,through which to find out the most suitable nutrient mediums for germination,proliferation and differentiation,rootage of Cymbidiummastersii Griff, seeds.The results showed that the nutrient medium for germination was 1/2MS＋6-BA 0.3 mg/L ＋ NAA 0.2 mg/L＋ pineapple juice（CM） 50 mL/L;the nutrient medium for protocorm was 1/2 MS＋6-BA 0.4 mg/L＋NAA 0.2 mg/L＋ AC 0.5 g/L;the nutrient medium for rootage was 1/2 MS＋NAA 0.5 mg/L＋ AC 2 g/L.