中文摘要：

从 Candida sp.99-125 的白手起家的脂肪分解酵素准备通过脂肪酸的酶的酯化作用被用于简历柴油机的生产。粗略的脂肪分解酵素粉末和发酵肉汤在一个便宜纤维布搬运人上被使不能调动。条件在在一个溶剂系统的长链的脂肪酸和甲醇之间的 催化oflipase 的酯化作用我们 reinvestigated 详细包括温度， 最适PH 值，底层集中，溶剂，吸收剂代理人，酶剂量和纯净，固定方法，增加 ofsubstrate.The 的模式结果显示出那反应温度，脂肪分解酵素微型环境，底层集中，酶剂量和纯净的 最适PH 强烈影响酯化作用。为改进包含加工费的酶的反应的几个新方法和酶的过程也被讨论，例如象反应溶剂的石块柴油，固定方法，甲醇的 multi-stepgradient 增加。92.8% 的酯化作用度在 12.5 h 反应周期以后在最佳的反应条件下面与油酸和甲醇被获得。从为酯化作用的粗略的免费脂肪分解酵素粉末的使不能调动的脂肪分解酵素准备的半衰期是 15 天。

英文摘要：

A self-made lipase preparation from Candida sp. 99-125 was used for the production of biodiesel through enzymatic esterification of fatty acids. The crude lipase powder and fermentation broth were immobilized on a cheap fiber cloth carrier. The conditions of lipase-catalyzed esterification between long-chain fatty acids and methanol in a solvent system were investigated in detail, including the temperature, pH value, substrate concentration, solvent, absorbent agent, enzyme dosage and purity, immobilization method, the mode of addition of substrate. The results show that reaction temperature, pH of lipase micro-environment, substrate concentration, enzyme dosage and purity affect the esterification strongly. Several new methods and enzymatic procedures for improving the enzymatic reaction involving the process cost are also discussed, such as fossil diesel fuel as reaction solvent, immobilization method, multi-step gradient addition of methanol. The esterification degree of 92.8% was obtained with oleic acid and methanol under the optimal reaction condition after 12.5 h reaction time. The half-life of the immobilized lipase preparation from crude free lipase powder for esterification was 15 days.