中文摘要：

利用高糖选择性富集培养技术,从天然高糖环境蜂巢和蜂蜜中筛选到99株耐高糖酵母。随后,对随机挑选出的40株分别来自不同分离源的菌株做了摇瓶发酵试验,并且对发酵培养液中的多元醇种类进行了初步鉴定分析,确立了一种简单有效分离底物葡萄糖和多元醇类产物的层析方法,即以微晶纤维素板为展开介质,定性检测的展开剂及其配比为：乙酸乙酯∶吡啶∶冰醋酸∶水=16∶10∶2∶3。实验结果显示,筛选到的菌株以产D-阿拉伯糖醇和甘油的居多。根据形态学观察、生理生化试验和ITS序列的测定分析对试验菌株进行鉴定。鉴定结果表明,所筛选的菌株可被归为3种酵母菌,分别为Lodderomyces elongisporus,Zygosaccharomyces rouxii,Starmerella sp.。通过筛菌实验得到1株高产D-阿拉伯糖醇的菌株Z.rouxii JM-12,经气相色谱法定量分析,得到D-阿拉伯糖醇的产量为（61.33±0.49）g/L,实验结果为进一步研究产D-阿拉伯糖醇耐高渗酵母奠定了基础。

英文摘要：

By a screening method with high concentration of D-glucose in the medium, 99 osmophilic yeasts were isolated from crude honeycomb and honey. Then, 40 strains isolated from different samples were fermented in flask- shaking to study the variety of polyol in the fermentation broth by TLC （Thin Layer Chromatography） analysis meth- ods. An effective method was established in this study to separate D-glucose and polyol. The qualitative detection of D-arabitol and byproducts was performed by TLC using a developing solvent system of normal ethyl acetate/pyridine/ glacial acetic acid/water （16： 10： 2： 3） on microcrystalline cellulose plate. Experimental results showed that most of these yeasts were D-arabitol-producing and glycerol-producing strains. According to the observation and analyses of morphological, physiological and biochemical characteristics, ITS sequences and the established phylogenetic tree, the three different colonies were identified as Lodderomyces elongisporus, Zygosaccharomyces rouxii and Starmerella sp.. A high-performing strain was isolated by screening tests, and the yield of D-arabitol was 61.33 ± 0.49 g/L, which detected by gas chromatography. The above results laid a foundation for further study on D-arabitol production by yeast.