欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
中药五倍子是一种具有高药用价值的漆树科植物的寄生虫瘿。本研究利用高速逆流色谱技术分离得到五倍子的7种组分,分离溶剂体系为：乙酸乙酯-乙醇-水（5∶1∶5,v/v/v）。利用微量热仪测定五倍子各分离组分对弧菌G1-1的抑制曲线,通过细菌生长速率常数和药物浓度之间的关系拟合公式,计算出各组分对菌株G1-1的最低抑菌浓度。结果表明,五倍子各分离组分均对菌株G1-1具有一定抑制作用,其中组分P5对弧菌G1-1的抑制效果最佳,最低抑菌浓度为0.10 mg/m L。该研究建立了一种分离制备中药材有效组分和筛选评价其生物活性的方法,为中药有效组分应用于防控水产动物细菌性疾病提供理论依据及参考。
英文摘要：
Rhus chinensis Mill is a kind of Chinese medicine,which is parasitic gallnut in Anacardiaceae plants. In this study,high-speed counter-current chromatography was successfully applied to separate 7 active fractions from ethanol extract of R. chinensis. The best two-phase solvent system consisting of ethyl acetate-ethanol-water（ 5∶ 1∶ 5,v / v / v） was determined by HSCCC. The antibacterial activity of 7 fractions on Vibrio G1-1 was investigated by microcalorimetry. The fitting equation of relationship between constant of bacterial growth rate and drug concentration were calculated for each fraction,and the minimal inhibitory concentrations were got. The results showed that each separated fraction had a certain inhibitory effect on Vibrio G1-1,in which P5 fraction showed the best inhibitory activity. The minimum inhibitory concentration of P5 fraction was 0. 10 mg / m L. This study established a useful method for the separation and preparation of active components from medicinal plants followed by the screening and evaluation of their antibacterial activity. It provided theoretical basis and reference to prevent and control bacterial disease in aquatic animal with Chinese medical plants.
同期刊论文项目
同项目期刊论文
期刊信息
位置：