中文摘要：

用华北地区流行的白粉菌15号生理小种，感染强抗白粉病的栽培小麦Brock和对白粉病敏感的小麦京411，通过蛋白质组技术分析其差异蛋白。结果表明，Brock经白粉菌感染12h后，至少有6个蛋白质斑点（43kD／pI 6．7、43kD／pI 6．9、43kD／pI 7．2、28kD／pI 5．8、26kD／pI 5．5和26kD／pI 6．5）表达量明显增加；感染3d后，有5个蛋白质斑点（48kD／pI 5．6、43kD／pI 6．9、43kD／pI 7．2、28kD／pI 5．8和26kD／pI5．5）表达量增加；感染5d后，有12个新的蛋白质斑点（16kD／pI7．6、42kD／pI6．5、40kD／pI4．8、40kD／pI4．6、31kD／pI5．7、16kD／pI4．6、20kD／pI8.3、50kD／pI6．7、48kD／pI6．6、28kD／pI5．7、23kD／pI4．8和25kD／pI4．7）被诱导合成，2种蛋白质斑点（26kD／pI4．6和17kD／pI7．9）消失。京411经白粉菌感染12h后，3个蛋白质斑点（21kD／pI6．4、18kD／pI5．4和14kD／pI7．0）表达量增加；感染3d后，有2个蛋白质斑点（80kD／pI5．4和14kD，pI7．0）表达量增加，1个蛋白质斑点（16kD／pI5．4）表达量下降；感染5d后，有3个蛋白质斑点（50kD／pI7.3、40kD／pI7．3和24kD／pI7．2）表达量增加，2个斑点（40kD／pI4．8和14kD／pI7．2）表达量下降，但没有发现新的蛋白质合成。对Brock中诱导产生的12个新蛋白质斑点，利用MALDI-TOF—MS方法，于NCBI进行数据查询，其中有6个分别属于F-box亮氨酸高度重复蛋白、重金属转运／解毒蛋白、β-1，3-葡聚糖酶（两个同工体）、β-1，3-葡聚糖酶前体、锌指蛋白。功能查询表明，上述6个蛋白参与细胞周期调控、发育、激素响应、基因转录和病害防御等。推测Brock和京411感染白粉菌后，出现的蛋白质组变化可能与各自的抗、感白粉病特性有关。

英文摘要：

The protein of resistant wheat （Triticum aestivum L.） variety Brock and susceptible variety Jing 411 was extracted from leaves and separated using two-dimensional polyacrylamid gel electrophoresis （2-DE） at 12 h, 3 d, and 5 d after inoculating prevalent race No. 15 orB. graminis f. sp. tritici. In Brock, compared with control with no pathogen inoculation, at least six pro- tein spots of 43 kD/pI 6.7, 43 kD/pI 6.9, 43 kD/pI 7.2, 28 kD/pI 5.8, 26 kD/pI 5.5 and 26 kD/pI 6.5 obviously increased in content at 12 h time point; and five protein spots of 48 kD/pI 5.6, 43 kD/pI 6.9, 43 kD/pI 7.2, 28 kD/pI 5.8 and 26 kD/pI 5.5 increased in content at 3 d time point. At 5d after inoculation, 12 novel proteins were induced, viz. 16 kD/pI 7.6, 42 kD/pI 6.5, 40 kD/pI 4.8, 40 kD/pI 4.6, 31 kD/pI 5.7, 16 kD/pI 4.6, 20 kD/pI 8.3, 50 kD/pI 6.7, 48 kD/pI 6.6, 28 kD/pI 5.7, 23 kD/pI 4.8 and 25 kD/pI 4.7; simultaneously, two protein spots that were observed earlier disappeared. In Jing 411, three protein spots of 21 kD/pI 6.4, 18 kD/pI 5.4, and 14 kD/pI 7.0 increased in content at 12 h after inoculation. At the 3 d time point, two protein spots of 80 kD/pI 5.4 and 14 kD/pI 7.0 increased in content, however, one protein spot （16 kD/pI 5.4） showed decrease in protein abundance. At the 5 d time point, three protein spots of 50 kD/pI 7.3, 40 kD/pI 7.3, and 24 kD/pI 7.2 and two protein spots of 40 kD/pI 4.8 and 14 kD/pI 7.2 showed increase and decrease in protein abundance, respectively, but there were no novel protein spots induced. Among the 12 novel protein spots induced in Brock, six spots were identified using MALDI-TOF-MS and NCBI database searching, which were F-box and leucine-rich repeat protein, heavy metal transport/detoxification protein, endo-beta-1,3-glucanase （two isozymes）, beta-1,3-glucanase precursor and zinc finger protein. These proteins are involved in a wide range of physiological processes, such as cell cycle control, development, phytohormone response, and resistance to fungal disease. Thus, the pro