中文摘要：

目的通过聚乙二醇（PEG）将YPSMA—l单克隆抗体（mAb）和聚酰胺一胺（PAMAM）连接，合成新型的前列腺癌靶向基因载体PAMAM—PEG—mAb，提高基因的转染效率和前列腺癌细胞靶向性。方法运用NMR法鉴定合成的PAMAM—PEG—mAb裁体的结构，在前列腺癌细胞（PC3和LNCaP）上进行摄取实验和基因转染实验考察载体的生物学性质。结果通过结构鉴定确定PAMAM—PEG—mAb合成成功。细胞摄取实验表明，PAMAM—PEG—mAb的细胞摄取效率呈浓度依赖性。载基因后体外实验表明，PAMAM经PEG修饰后转染效率和前列腺癌靶向性无明显变化，再经mAb修饰后LNCaP细胞显著增加，而PC3细胞反而有下降。结论PAMAM—PEG—mAb载体是一种有潜力的前列腺癌靶向基因转运载体。

英文摘要：

OBJECTIVE Aim to construct a novel polymer gene delivery system based on a new kind of dendrimer-poly- amidoamine （PAMAM）. With the modification of polyethylene glycol （PEG） and YPSMA-1 monoclonal antibody （mAb）, PAMAM- PEG-mAb was successfully synthesized as a novel gene vector targeting to the prostatic cancer. METHODS NMR was used to char- acterize PAMAM-PEG-mAb. The cellular uptake and prostatic cancer （PCa） distribution experiments were employed to explore its bio- logical characteristics and PCa cell （ PC3 and LNCaP） targeting ability. RESULTS NMR results demonstrated the successful synthesis of PAMAM-PEG-mAb. The cellular uptake of vectors was concentration-dependent. The ~ene expression in vitro indicated that themodification of mAb could increase the gene expression efficiency and PCa targeting ability of PAMAM vectors to LNCaP （ PSMA overex- pressing prostate cancer cells）. CONCLUSION PAMAM-PEG-mAb is a potential gene delivery vector targeting to PCa.