中文摘要：

为探讨As2O3诱导MD肿瘤细胞株凋亡的机制，采用MTT法检测As2O3对MDCC—MSB1的抑制作用，采用荧光显微镜观察细胞形态学变化，DNALadder法检测细胞凋亡，Fura-2／AM负载双波长荧光分光光度计检测细胞内游离钙离子浓度（[Ca^2＋]i）变化，观察As2O3对鸡MD肿瘤细胞株MDCC—MSB1的影响。结果显示：As2O3能抑制鸡MD肿瘤细胞株MDCC—MSB1的生长，呈剂量依赖关系（P〈0．05或P〈0．01）；在荧光显微镜下可见As2O3作用后MDCC-MSB1细胞呈现典型的凋亡特征，并出现典型的DNA Ladder，双波长荧光分光光度计检测结果显示：As2O3作用后的MDCC-MSB1细胞内[Ca^2＋]i升高（P〈0．05或P〈0．01），呈剂量依赖关系。As2O3能明显抑制鸡MD肿瘤细胞株MDCC—MSB1细胞的生长，并诱导其发生凋亡，细胞内[Ca^2＋]升高可能是其诱导细胞凋亡的机制之一。

英文摘要：

To explore the mechanism of MDCC-MSB1 apoptosis induced by Arsenic Trioxide （As2Os）,inhibition percentage was detected by MTT assay;morphology changes was examined by fluorescence microscope;apoptosis was examined by DNA Ladder; [Ca^2＋]i was investigated by spectrofluorimeter on MDCC-MSB1 cells. The results were follows： As2O3 inhibited the proliferation of MDCC-MSB1 cells in concentration dependent manner （P〈0.05 or P〈0.01）; typical apoptosis character was seen by fluorescence microscope;DNA Ladder were observed;spectrofluorimeter analysis showed that the [Ca^2＋]i was elevated significantly after the treatment of As2O3 （P〈0.05 or P〈0.01）and showed relationship of concentration dependent. As2Os can induce MDCC-MSB1 apoptosis. The increase of [Ca^2＋]i may be one of its mechanisms.