中文摘要：

将层层自组装技术与仿生矿化技术相结合, 由聚苯乙烯磺酸钠、聚二甲基二烯丙基氯化铵和二氧化硅成功制备（聚苯乙烯磺酸钠-聚二甲基二烯丙基氯化铵）2-二氧化硅复合微囊. 采用扫描电子显微镜、红外光谱和热重对微囊的形貌和化学结构进行了表征. 以该复合微囊作为理想载体固定化葡萄糖氧化酶. 结果表明, 固定于复合微囊中的葡萄糖氧化酶的热稳定性、pH稳定性、操作稳定性得到了提高; 在最适条件下, 复合微囊固定化葡萄糖氧化酶的酶活回收率为72.85%, 米氏常数是游离葡萄糖氧化酶的2.21倍. 复合微囊在化学/生物催化、药物/基因传递系统和生物传感器应用方面具有一定的潜能.

英文摘要：

Organic‐inorganic composite microcapsules made of sodium poly（styrenesulfonate）（PSS）,poly（dimethyl diallyl ammonium chloride）（PDADMAC）,and biomimetic silica were successfully fabricated via a combination of layer‐by‐layer self‐assembly and biomimetic mineralization.The morphologies and chemical compositions of the microcapsules（denoted by（PSS/PDADMAC）2‐SiO2）were characterized using scanning electron microscopy,Fourier‐transform infrared spectroscopy,and thermogravimetric analysis.The composite microcapsules were used as carrier for the immobilization of glucose oxidase（GOD）.GOD encapsulated in（PSS/PDADMAC）2‐SiO2（denoted by GOD@（PSS/PDADMAC）2‐SiO2）displayed improved thermal,pH,and operational stabilities.The activity recovery of the immobilized GOD reached 72.85% under the optimized conditions.GOD@（PSS/PDADMAC）2‐SiO 2 showed a 2.21‐fold increase in the Michaelis constant（Km） compared with free GOD.The composite microcapsules have the potential for a range of applications in chemical/biological catalysis and drug/gene delivery systems,and as biosensors.