中文摘要：

通过使用不同的培养基，采用液体培养法研究了蔗糖、硼酸和钙离子对二乔木兰花粉萌发的影响。将所用花粉分为两组，组1于8：00采集，组2于16：00采集，结果表明：一定浓度的蔗糖、硼酸和钙离子对花粉的i蒴发有较大的促进作用，但超过一定浓度时则起抑制作用，二乔木兰花粉萌发的最适培养基为30g·L^-1蔗糖＋100mg·L^-1硼酸＋500mg·L^-1Ca^2＋，在该培养基内，组1花粉的萌发率为46．21％±1．86％，花粉管长度为1156．25μm；组2花粉的萌发率仅为14．22％±1．86％，花粉管长度为1022μm。此外，在其离体萌发过程中，二乔木兰花粉存在花粉管双萌发的现象。用不同的花粉保存方法保存二乔木兰花粉后，发现干燥处理后于-18℃下保存的花粉活性最高，30d后仍有6．92％±0．75％的萌发率，而于常温25℃下保存的花粉10d后就基本失活，说明低温干燥保存是保存二乔木兰花粉活性的有效途径。

英文摘要：

Study on effect of sucrose, boric acid and calcium ion on germination of Magnolia soulangeana pollen by 19 kinds of culture medium. Pollen grains were collected at 8：00 as group 1 and collected at 16：00 as group 2. The result showed that sucrose, boric acid and calcium ion could promote pollen germination and tube growth at certain concentration. The optimal medium was 30 g ·L^-1 sucrose ＋ 100 mg ·L^-1H3BO3 ＋ 500 mg·L^-1CaC12 · 2H20 with 46.21% ±1.86% and 1 156.251am pollen germination rate and tube length of group 1, with only 14.22% ± 1.86% and 1 0221xm of group 2. Two pollen tubers from pollen were observed during in vitro germination. Study on storage conditions of M. soulangeana pollen resulted that the pollen stored at 18℃ after drying could have survival rate of 6.92% ± 0.75% after 30 days. But that stored at 25℃ died 10 days later. It demonstrated that pollen grains of the plant should be stored at conditions with low temperature after drying.