中文摘要：

对中国广泛分布的黑翅土白蚁（Odontotermes formosanus）头部内切β-1,4葡聚糖酶（endo-beta-1,4-glucanase）基因进行克隆,并在原核生物大肠杆菌体内进行了表达.根据Genbank上已报道的endo-beta-1,4-glu-canase基因设计引物,通过RT-PCR获得1个完整的内切β-1,4葡聚糖酶基因.通过基因测序以及基因比对发现与Genbank上发表的内切β-1,4葡聚糖酶同源性高达96.40%.将获得的基因导入大肠杆菌体内进行诱导表达,表达出的蛋白经Western-blot检测,为该基因所表达的目的蛋白.实验为进一步对内切β-1,4葡聚糖酶的实际应用奠定一定的实验基础.

英文摘要：

Endo-beta-1,4-glucanase gene,which was expressed in the head of Odontotermes formosanus wildly distributed in China,cloned and expressed in E.coli bacteria.A pair of specific primers of the endobeta-1,4-glucanase gene,which was designed according to Genbank database,was used to amplify the complete endo-beta-1,4-glucanase gene by RT-PCR technology.Through gene sequencing and comparing with the endo-beta-1,4-glucanase gene publicated in the Genbank database,the homology of the endo-beta-1,4glucanase gene in the research achieved as high as 96.40%.The endo-beta-1,4-glucanase gene was inserted to the prokaryotic expression system,and induced to translate into protein in the suitable condition.The expression protein was analysed by Western-blot,the result indicated that the endo-beta-1,4-glucanase gene was translated into the objective protein.This research is an experimental foundation for the advanced application study of the endo-beta-1,4-glucanase.