中文摘要：

目的探讨胰岛素和GF-Ⅱ与结直肠癌发生、发展的关系及生物学意义。方法用免疫组化法检测胰岛素在人结直肠腺瘤和结直肠癌中的表达情况;用免疫印迹（Western Blotting）方法检测IGF-Ⅱ在人结直肠腺瘤和结直肠癌中的表达。结果胰岛素在人正常结直肠粘膜及结直肠腺瘤中基本不表达,在结直肠癌中高表达,与正常对照组比较,P〈0.01,在Ducks分期的各阶段中表达无差异。IGF-Ⅱ在正常结直肠粘膜中有微量表达,在结直肠腺瘤中的表达较正常结直肠粘膜增加,与正常对照组比较,P〈0.01,而在结直肠癌中则高表达,与正常对照组及腺瘤组比较,P〈0.01,其在结直肠癌中的表达与Duck分期无显著差异。结论胰岛素在人结直肠癌中高表达;IGF-Ⅱ在人结直肠腺瘤及结直肠癌中均有表达,在结直肠癌中表达更高。这说明胰岛素、IGF-Ⅱ与结直肠癌发生、发展有关。

英文摘要：

Objective To investigate whether insulin and insulin-like growth factor-II（IGF-II） were involved in colorectal carcinoma aetiologically and pathologically.Methods Immunohistochemistry and western blot methods were respectively employed to examine the expression of the two factors in human colorectal adenoma and colorectal carcinoma.Results Insulin was almost undetectable in normal colorectal mucosa and colorectal adenoma,while over expressed in colorectal carcinoma at the same level during all Duck stages as indicated by immunohistochemistry.Low level IGF-II was blotted in normal colorectal mucosa;IGF-II expression level was higher in colorectal adenoma（p0.01）,and the highest in colorectal carcinoma.Likewise,no difference was observed during all Duck stages.Conclusions Insulin was overexpressed in colorectal cancer.IGF-Ⅱwas also expressed in colorectal adenoma and colorectal carcinoma,which was higher in colorectal adenoma than that in colorectal carcinoma.The results suggest that insulin and IGF-II might contribute to the occurrence and development of colorectal carcinoma.