中文摘要：

RUB1（related to ubiquitin 1）是植物和酵母中一种泛素类似蛋白质,是Cullin家族的一个成员。为阐释津田芜菁Br RUB1基因的表达特性,本研究克隆了津田芜菁RUB1基因的全长c DNA序列,命名为Br RUB1,Gen Bank登录号为KF501173。其ORF全长471 bp,编码156个氨基酸;亚细胞定位结果显示,Br RUB1-GFP定位于细胞核,表明Br RUB1蛋白可能在细胞核中发挥其功能。q RT-PCR检测Br RUB1的表达表明,该基因表达量在花蕾中最高,花瓣中次之,具有组织特异性。而且Br RUB1在芜菁根皮中的表达受长波紫外线（UV-A）诱导。

英文摘要：

RUB1（related to ubiquitin 1） is a ubiquitin-like protein in plant and yeast, belong to Cullin family. To elucidate the expression feature of RUB1 in Tsuda, we isolated c DNA of RUB1 gene from this plant and named Br RUB1（Gen Bank accession No. KF501173）. The c DNA of Br RUBI was 670 bp in full length and its open reading frame（ORF） was 471 bp in full length, encoding 157 amino acids. Br RUB1-GFP was localized to nucleus, indicating that Br RUB1 may play an important role in the nucleus. q RT-PCR analysis showed that Br RUB1 expressed highly in bud and less in petal, showing tissue specificity. Furthermore, the expression of Br RUB1 was induced by UV-A light in the swollen hypocotyls.