中文摘要：

目的：观察橙皮苷对人原代黑素细胞酪氨酸酶及黑素合成的影响，探讨其作用机制。方法：选择50、100、200、300、400、500和600mg／L7个浓度的橙皮苷作用于体外培养的人表皮黑素细胞72h，分别测定细胞增殖活性、酪氨酸酶活性及黑素含量。结果：橙皮苷显著抑制黑素合成和酪氨酸酶活性，且呈浓度依赖性。结论：橙皮苷可抑制黑素合成和酪氨酸酶活性。

英文摘要：

Objective： To observe the effects of hesperidin on melanin synthesis and tyrosinase activity. Methods： Normal human melanoeytes were isolated from human foreskin. Melanocytes were incubated with the following concentrtions of hesperidin： 50, 100, 200, 300, 400, 500 and 600mg/L for 72h, respectively.The rate of proliferation, melanin content, and tyrosinase activity were measured. Results： The results showed that hesperidin treatment inhibited melanin synthesis and tyrosinase activity significantly compared with the control. Hesperidin decreased the cellular melanin content in dose dependent manner. Conclusion： Hesperidin can inhibit melanogenesis and the activity of tyrosinase, which may be induced by down regulation of tyrosinase.