中文摘要：

目的进一步评价丹参酮ⅡA纳米结构脂质载体（TⅡA-NLC）的性质,并考察其对HaCaT细胞增殖的影响。方法采用纳米粒度仪及HPLC法对TⅡA-NLC的粒径和光稳定性进行考察,透析袋法测定TⅡA-NLC在72 h内的累积释放量并绘制释放曲线,MTT法考察TⅡA-NLC对HaCaT细胞增殖的影响。结果 TⅡA-NLC的平均粒径为（178±9）nm,多分散度指数（PDI）为0.183±0.017,Zeta电位（-27.5±5.6）m V,体外72 h累积释放量为52.28%,TⅡA-NLC能显著降低TⅡA的光降解速率,TⅡA在一定范围内以剂量依赖性的方式抑制HaCaT细胞增殖,同质量浓度的TⅡA-NLC对HaCaT细胞增殖的抑制作用显著高于TⅡA溶液。结论 TⅡA-NLC稳定性好,具有良好的缓释作用,较好的细胞生物相容性,能显著提高TⅡA对HaCaT细胞增殖的抑制作用。

英文摘要：

Objective To evaluate TⅡA-NLC＇s quality in vitro and study its effects on the HaCaT cells. Methods Its particle size, polydispersity index（PDI） and photostability were investigated by Zetasizer analyzer and high-performance liquid chromatography（HPLC）. The in vitro release of TⅡA-NLC within 72 h was measured by dialysis method; Meanwhile MTT assay was used to detect proliferation activity on HaCaT cells. Results The average particle size, PDI and Zeta potential of TⅡA-NLC was：（178 ± 9） nm, 0.183 ± 0.017 and（-27.5 ± 5.6） m V. The 72 h in vitro accumulative release rate was 52.28%, and the degradation velocity of TⅡAwas significantly slowed down. The TⅡA-NLC inhibited the proliferation of HaCaT cells in concentration-dependent mode within a certain range. Compared with TⅡA group, TⅡA-NLC had more effects on the HaCaT cells. Conclusion The prepared TⅡA-NLC showed good stability, sustained release of interest, good cell biology intermiscibility, and could obviously increase the effects on the HaCaT cells.