中文摘要：

为分析流行于信鸽群中新城疫病毒致病株P／Anhui／I／09的分子遗传特性及其囊膜糖蛋白的差异性，采用RT-PCR法扩增其F和HN基因开放阅读框（ORF），并与已公布的主要代表性毒株序列进行遗传进化分析及615个全长F基因和480个全长HN基因推导氨基酸序列比对分析，同时对P／Anhui／I／09株进行型内遗传关系分析。结果显示：P／Anhui／I／09株F和HN基因开放阅读框长度分别为1662bp和1716bp，GenBank登录号分别为JQ290284和JQ305097，其F基因与NDV05—029、PG／CH／JS／I／06、CH／98-1鸽源毒株亲缘关系最近，同源率在98．2％～99．5o／01同属基因vm亚型，但与传统疫苗株LaSota、V4、B1和Mukteswar的遗传距离较远，同源率仅在84％～86．3％。ⅣⅣ基因ORF进化树分支拓扑结构与F基因相似。型内遗传关系分析显示，VIb亚型可进一步为VIb1～VIb55个进化分支，其中我国分离株都位于Vlbl和VIb4进化分支中。氨基酸序列比对及糖基化位点分析显示，P／Anhui／I／09的F蛋白裂解位点氨基酸序列为^112K．R—Q—K—R．F^117，与NDV强毒株分子特征相符，并发现在七肽重复区旁497位新增一个潜在N一糖基化位点，除2个基因III型日本分离株（Miyadera和MIY／51）外，所有含497位新增糖基化位点的毒株都位于VIbl分支。包含P／Anhui／I／09株在内的大部分鸽源毒株在F蛋白aa132S（融合肽区）、aa259H和HN蛋白aa3H（胞质区）、aal22R、aa347G、aa349E存在一定特征性。

英文摘要：

To analyze the molecular genetic characteristics and variability of envelope glycoprotein of pathogenic strain of Newcastle disease virus P/Anhui/1/09 epidemic in pigeon flock, the sequences of open read- ing frame （ORF） of F and HN gene were amplified by RT-PCR. Evolution analysis was performed with the repre- sentative strains and P/Anhui/1/09. The published 615 F full-length gene and 480 HN full-length gene were de- duced to amino acid sequence, and then were analyzed. Genetic relationship of strains from genotype VI contain- ing P/Anhui/1/09 was analyzed. The results showed that F and HN genes ORF length of P/Anhui/1/09 were 1 662 bp and 1 716 bp, and their GenBank accession number were JQ290284 and JQ305097, respectively. P/Anhui/1/09 was closest to NDV05-029, PG/CH/JS/1/06, CH/98-1 strains of pigeon-origin based on F gene belonged to VIb subtype, and their homologous rates were from 98.2% to 99.5%. However, it was fruther to the traditional vaccine strains of LaSota, V4, B 1 and Mukteswar with 84% to 86.3% in homology. Phylogenetic tree topology of the HN gene ORF was similar to its F gene ORF. VIb subtype could be divided into five evolutionary branches as VIb 1-VIb5 based on genetic relationships, and strains belonged to VIb subtype isolated from China were all lo- cated in VIbl and VIb4 branches. The F protein cleavage site amino acid sequence of P/Anhui/1/09 was ll2K-RQKR-Fll7 based on amino acid sequence analysis, which was consistent with virulent strains of NDV. A new potential N-glycosylation site 497 adjacent heptad repeat region was discovered in strains belonged to VIbl branch containing P/Anhui/1/09 except two genotype III strains （Miyadera and MIY/51） isolated from Japan. There existed a certain characteristic on F protein aa132S （region of fusion peptide）, aa259H, and HN protein aa3H （cytomere）, aa122R, aa347G, aa349E at most strains of pigeon-origin containing P/Anhui/1/09.