中文摘要：

目的：探讨不同时间持续缺氧孵育后脂肪细胞脂联素和瘦素表达的变化。方法：将分化成熟的3T3-L1脂肪细胞随机分为6组，即常氧（21％O：）组和缺氧（1％O，）4、12、24、48、72h组，实时定量PCR（qRT—PCR）法测定各时间点脂肪细胞中缺氧诱导因子let（HIF-1α）、葡萄糖转运子1（GLUT-1）、脂联素和瘦素基因表达。结果：与常氧组相比，缺氧12、24、48h组HIF-1α、GLUT-1mRNA表达水平升高，差异有统计学意义（P〈0．05）。缺氧24h时HIF-1α、GLUT-1mRNA达到最高。各缺氧组脂联素mRNA在12、24、48、72h缺氧组的表达下降，较常氧组有统计学差异（P〈0．05）。各缺氧组瘦素mRNA的表达高于常氧组，并在24h时达到最高，差异有统计学意义（P〈0．05）。结论：缺氧使脂肪细胞HIF-1α、GLUT-1、瘦素mRNA表达升高，脂联素mRNA表达下降，各反应在缺氧24h时达到最大水平。

英文摘要：

Objective： To explore the effect of different incubation time of hypoxia on the expression of adipokines in the 3T3-L1 adipocytes. Methods： The differentiated 3T3-L1 adipocytes were randomly divided into 6 groups, normoxia （21%O2）and hypoxia （1%O2） conditions for 4, 12, 24, 48, 72 h respectively. The mRNA expressions of hypoxia inducible factor-1α （HIF-1α）, glucose transporter 1 （GLUT-1）, adiponectin and leptin were detected by the qRT-PCR. Results： In 3T3-L1 adipocytes, the mRNA expressions of HIF-1α and GLUT-1 in hypoxia conditions for 12 h, 24 h and 48 h were higher than normoxia-treated cells （P〈0.05）; furthermore, they were highest in the group of hypoxia-treated cells for 24 h. Compared with the normoxia group, the adiponectin mRNA expressions of the hypoxia-treated 3T3-L1 adipoeytes decreased in all groups （P〈0.05） except for the 4 h group （P〈0.05）. Ttranscription level of leptin in the hypoxia-treated cells increased（P〈0.05）, and it reached the highest level in the 24 h hypoxia group. Conclusion： The mRNA expressions of HIF-1α, GLUT- 1 and leptin of 3T3-L1 adipocytes increase in the condition of hypoxia incubation, while the level of adiponectin mRNA decreases under hypoxia treatment. All changes above reach the peaks within the 24 h of hypoxia-treated 3T3-L1 adipocytes.