中文摘要：

目的：探索鼻腔NK／T细胞淋巴瘤（N-NK／T-L）中淋巴细胞发育相关基因的表达及意义。方法：通过对EOMES，ETS-1和MEF基因进行克隆、探针制备和组织芯片制备，对25例N-NK／T-L以及同部位11例B细胞淋巴瘤（BCL）、6例T细胞淋巴瘤（TCL）、3例正常脾组织和5例慢性炎性鼻黏膜组织进行了原位杂交检测。结果：EOMES、ETS-1、MEF基因在N-NK／T-L、BCL、TCL、脾和鼻粘膜中均有表达，但阳性率和表达强度不同。EOMES基因在N-NK／T-L的表达强度与BCL和TCL间有差异，MEF基因在N-NK／T-L的表达阳性率和强度与TCL间有差异，而ETS-1基因在N-NK／T-L与对照组间的表达无差异。T-bet、EOMES和MEF基因在N-NK／T-L的表达具有相关性。结论EOMES和MEF基因在N-NK／T-L中高表达，可能在该肿瘤发生和组织坏死中起重要作用，而ETS-1基因在N-NK／T-L和对照组中普遍表达，提示其可能在淋巴造血系统的发育和功能中起基础性的共同通路。

英文摘要：

Objective：To explore the expression and significance of the genes related to lymphocyte development in nasal NK/T lymphoma （N-NK/T-L）. Methods：The expression of EOMES （eomesodermin homolog） gene, ETS-1 gene and MEF （myeloid Elf-1 like factor） gene in 25 cases of N-NK/T-L and 25 control cases （including 11 cases of B cell lymphoma （BCL）, 6 cases of T cell lymphoma （TCL）, 3 cases of normal spleen, 5 cases of chronic nasopharyngitis） were detected by tissue microarray and in situ hybridization. Results：Three genes each have its expression in N-NK/T-L and control groups. EOMES gene expressed in all N-NK/T-L and 84O/o of them were high expression. Compared with BCL （55% high expression） and TCL （17% high expression）, the expression of EOMES in N-NK/T--L was much higher than that in BCL （P=0.0029） and TCL （P=0.0010）. MEF gene expressed in 24 （96%） of 25 cases N-NK/T-L and 17 cases （68%） were high expression, and its expression in N-NK/ T-L was significandy higher than that in TCL （P=0.0050）, in which only 1 case （17%） was high expression. ETS-1 expressed in all cases of N-NK/T-L and control groups. Conclusion：The expression of EOMES gene and MEF gene may function in the development of N-NK/T-L and the tumor necrosis. The general expression of ETS-1 gene indicates that it may be a fundamental common part of the program of lymphoid development and function.