中文摘要：

通过分别向匍枝筋骨草悬浮培养体系中添加不同浓度的脱落酸（ABA）并测定添加后不同时间悬浮细胞的生长状况和生物量,以及蜕皮甾酮（20E）和ABA的浓度变化,筛选出最适宜添加浓度.通过测定悬浮细胞内玉米素（ZR）、赤霉素3（GA3）和吲哚乙酸（IAA）及20E的含量变化,探讨ABA对匍枝筋骨草悬浮细胞生长的影响.结果表明,添加0.15 mg·L^-1ABA后细胞生长速度减缓,但在未褐化期ABA促进20E的累积,在第48 h和72 h悬浮细胞中20E含量显著地高于对照组,但在第96 h悬浮细胞出现褐化现象,20E含量下降极显著地低于对照组;向匍枝筋骨草悬浮体系中加入外源ABA,悬浮细胞内ABA含量呈上升趋势,ZR,GA3,IAA含量呈相对下降趋势;在相同条件下添加ABA抑制剂（Na2WO4）,则ABA,ZR,GA3和IAA的变化趋势与添加ABA相反,对20E的影响不显著.

英文摘要：

The optimal concentration of abscisic acid （ABA） was evaluated in this work by adding different concentrations of ABA into the suspension cell cultures system of Ajuga lobata D.Don.The growing status, biomass of suspension cultures cell, concentration of ecdysterone （20E） were measured after adding those substances.Upon the selection of optimal concentration, 0.15 mg·L^-1 ABA was added into Ajuga lobata D.Don suspension cell culture system, then changes of endogenous phytohormones, such as zeatin（ZR）, gibberellin 3（GA3）, Hetero auxin（IAA） and 20E content were determined with HPLC, and the impact of ABA on plant endogenous hormone content and 20E synthesis in suspension cultures cell of Ajuga lobata D.Don were analyzed.Adding ABA in the suspension cell culture system, we found that the growing speed of suspension cell was decreased, but without browning, ABA promoted the accumulation of 20E.At 48 h and 72 h after the addition of ABA, the content of 20E was significantly higher than that in the control group, and at 96 h after its addition, those treated with suspension cells became browning, and the dry weight of those cells was significantly lower than that of the control group.The content of endogenous ZR, GA3 and IAA in the treated suspension cell was also decreased.After the addition of Na2WO4 into the suspension cell culture system, the change trend of ABA, ZR, GA3 and IAA was opposite to that of adding ABA, and their range of 20E concentration change never reached to the significant difference level as compared with that of the control group.