中文摘要：

目的探讨三黄生肤油对糖尿病大鼠末梢循环障碍和足溃疡的作用及相关机制。方法（1）取25只Wistar大鼠按照随机数字表法分为非糖尿病组、糖尿病假治疗组、二甲双胍组、三黄生肤油组、联合治疗组，每组5只。非糖尿病组注射柠檬酸钠缓冲液，其余4组大鼠注射10mg／mL链脲佐菌素诱导建立糖尿病模型。注射后3周，5组大鼠均行足部低温处理制备末梢循环障碍模型。注射后第9—12周，非糖尿病组和糖尿病假治疗组大鼠均以生理盐水灌胃，双后足底涂抹麻油；二甲双胍组大鼠以稀释的二甲双胍灌胃，双后足底涂抹麻油；三黄生肤油组大鼠以生理盐水灌胃，双后足底涂抹三黄生肤油；联合治疗组大鼠以稀释的二甲双胍灌胃，双后足底涂抹三黄生肤油。注射后第9周治疗前（以下称治疗前）及治疗1、2、3周，采用红外测温仪及足底测试仪分别测定大鼠双后足底温度和热痛阈值。（2）另取25只大鼠同前分组并建立糖尿病模型（非糖尿病组除外），注射后第9周再行左后足底水蒸气烫伤制备足溃疡模型，并给予相应药物治疗。治疗3、7、21、35d，观察创面大体情况及面积变化。治疗35d处死大鼠，取创面组织行HE染色观察组织形态学及免疫组织化学染色检测环氧化酶2（COX-2）及血管内皮生长因子（VEGF）的表达。对数据行重复测量方差分析、单因素方差分析、Bonferroni事后检验。结果（1）糖尿病末梢循环障碍实验。除治疗2周时联合治疗组及治疗3周时二甲双胍组、三黄生肤油组、联合治疗组（t值为0．258～2．647，P值均大于0．05），各时相点4组糖尿病大鼠足底温度均明显低于非糖尿病组（t值为2．811—6．066，P值均小于0．05）。除治疗2、3周联合治疗组大鼠足底温度明显高于糖尿病假治疗组（t值分别为3．419和2．863，P值均小于0．05），各时相点二甲双

英文摘要：

Objective To observe the effects of San-huang-sheng-fu oil （S） on peripheral circulatory disorders and foot ulcers in diabetic rats and the relevant mechanisms. Methods （ 1 ） Twenty-five Wistar rats were divided into non-diabetes （N） , diabetes and sham treatment （DS） , metformin （ M ） , S, and combined treatment （CT） groups according to the random number table, with 5 rats in each group. Rats in group N were injected with sodium citrate buffer solution, while rats in the other 4 groups were injected with 10 mg/mL streptozotocin to induce diabetes. In post injection week （PIW） 3, feet of rats in all the 5 groups received an ice-cold stimulation to induce peripheral circulatory disorders. From PIW 9 to 12, rats in groups N and DS were gavaged with saline and applied with sesame oil on pelma of both hind limbs ; rats in group M were gavaged with diluted M and applied with sesame oil on pelma of both hind limbs; rats in group S were garaged with saline and applied with S on pelma of both hind limbs; rats in group CT were gavaged with diluted M and applied with S on pelma of both hind limbs. In PIW 9 before treatment （ hereinafter referred to as before treatment） and post treatment week （PTW） 1, 2, and 3, plantar temperature and hot pain threshold of rats were detected by infrared thermometer and foot tester respectively. （2） Another 25 rats were divided and induced with diabetes （expect for group N） as above. In PIW 9, rats in the 5 groups were inflicted with foot ulcer in the left pelma of hind limb by steam and received the corresponding treatment. On post treatment day （PTD） 3, 7, 21, and 35, the general condition and area of wounds were observed and measured respectively. All the rats were sacrificed on PTD 35, and wound tissue was collected for histomorphological observation and determination of expressions of cyclooxygenase-2 （COX-2） and vascular endothelial growth factor （VEGF） using HE staining and immunohistochemical staining respectively. Data were pr