中文摘要：

本研究通过线粒体分子探针标记技术检测孤雌激活早期胚胎线粒体的分布变化,运用实时荧光定量PCR技术检测mtDNA拷贝数的变化,揭示早期胚胎发育过程中线粒体分布、mtDNA拷贝数变化趋势。结果表明,成熟卵母细胞电激活后,由2-细胞胚胎开始,卵裂球内线粒体分布均匀且密集,每个细胞均有分布,卵裂球之外的空隙未见线粒体分布,直到囊胚形成,线粒体均有分布。孤雌激活4-细胞胚胎mtDNA拷贝数显著高于8-细胞胚胎mtDNA拷贝数（907210.77±145520.77,186224.33±103308.00,P〈0.05）,但显著低于2-细胞胚胎、桑椹胚、囊胚的mtDNA拷贝数（1563422.54±224666.51、1697626.25±176999.53和1752301.29±101146.64,P〈0.05）。孤雌激活扩张囊胚mtDNA拷贝数最高,为2812545.67±156819.31,显著高于其他发育时期胚胎的mtDNA拷贝数（P〈0.05）。由此可见,孤雌激活早期胚胎发育进程中线粒体分布及mtDNA拷贝数会发生变化。

英文摘要：

Mito-Tracker Green and Real-time quantitative PCR were used to respectively detect the mitochondria distribution and mtDNA copy number of porcine parthenogenetic oocytes. The results showed that the distribution of mitochondria was uniform and dense in the every blastomere beginning from 2-cell embryo. The outside of the space in blastomeres was no distri- bution of mitochondria. Until the blastocyst formation, mitochondria were distributed in blastomeres, mtDNA copy number of 4-cell embryos was significantly higher than that of 8-cell embryos （907210.77 4-145520. 77,186224. 33-＋-103308.00, P〈 0.05）, and it was significantly lower tharl those of Z-cell embryos, molura and blastocyst （1563422. 54 4-224666. 51, 1697626.25：1：176999.53 and 1752301.29：t：101146.64, P〈0.05）. mtDNA copy number of parthenogenetic expanded blastocyst was 2812545. 674-156819.31, which was the highest value of all different developmental embryos. In conclusion, mitochondrial distribution and mtDNA copy number changes could differ in the developmental process of parthenogenetic embryos.