中文摘要：

利用酒精沉淀结合氯仿和丁醇脱蛋白法，从南极海冰细菌Pseudoalteromonassp．Bsi20310发酵液中制备得到该菌株所产的胞外多糖（Exopolysaccharide，Bsi20310EPS）粗品。以Bsi20310EPS为助凝剂，可以明显改善铁盐对模拟水溶性染料活性艳红X-3B废水的混凝效果，在pH10左右，Fe（111）浓度0．98mmol·L^-1条件下加入150mg·L^-1Bsi20310EPS，脱色率由16％提高到84％。利用傅里叶变换红外光谱法（FTIR）分析比较Bsi20310EPS、Fe（Ⅲ）混凝剂-Bsi20310EPS絮体及Fe（Ⅲ）混凝剂一Bsi20310EPS-活性艳红X-3B絮体的官能团，图谱显示，Bsi20310EPS含有丰富的一0H、-COOH及糖苷键等活性基团；当Bsi20310EPS与Fe（Ⅲ）混凝剂作用后，3429cm。处尖峰变成宽峰，2921cm^-1处峰形减弱或消失，1650cm^-1处锐峰变成钝峰，1242cm_1处峰出现轻微红移，1151～1038cm。范围内杂多峰变成单一尖峰，表明-OH、-COOH及糖苷键是发生反应的主要官能团。研究结果预示着Bsi20310EPS可以作为一种安全有效的微生物助凝剂。

英文摘要：

Bsi20310 exopolysaccharide （Bsi20310 EPS） was secreted by a bacteria named Pseudoalteromonas sp. Bsi20310, isolated from Antarctic Sea ice. Crude Bsi20310 EPS was prepared by precipitation of the culture solution with ethanol, with proteins removed by using chloroform and butanol preparatorily. The results showed that Bsi20310 EPS improved the FeCI3 coagulation performance on synthetic watersolubte dye reactive red X-3B dyeing wastewater, obviously. The optimum coagulation enhancement of Bsi20310 EPS expressed by decolorizatxon rate is from 16% to 84%, at pH near 10, Fe（ Ⅲ） concentration of 0. 98 mmol·L^- 1 and Bsi20310 EPS concentration of 150 mg · L^-1 , respectively. Fourier transform infrared spectroscopy （FTIR） was used to investigate the functional groups of Bsi20310 EPS, Fe（ Ⅲ ）-Bsi20310 EPS floc and Fe（ Ⅲ）-Bsi20310 EPS-reaetive red X-3B floe. The spectra showed that Bsi20310 EPS contained a large number of functional groups such as --OH, --COOH and glycosidic bond. Some certain functional groups of Bsi20310 EPS changed being combined with Fe（ nI ） hydrolysate. For in-stance, narrow peaks at 3 429 and 1 650 cm-1 became wide; the peak at 2 921 cm 1 weakened or disappeared; the peak at 1 242 cm-1 red-shifted slightly; peaks in the region of 1 151-1 038 cm-1 became single and sharp, etc. The change in spectra indicated that --OH, --OOH and glycosidic bond might be the main functional groups. The study suggested a bright prospect of Bsi20310 EPS performing as an approach to safe and effective microbial coagulation enhancement.