中文摘要：

目的：观察电针干预对肥胖大鼠下丘脑DNA甲基转移酶1（Dnmt1）表达的影响。方法：将30只雄性SD大鼠随机分为对照组（n=10）和高脂组（n=20）,高脂组采用高脂饲料喂养12周制备食源性肥胖大鼠模型。将造模成功的大鼠随机分为针刺组（n=7）和肥胖组（n=7）。针刺组固定后取中脘、天枢、三阴交、后三里穴采用电针干预,5d为1个疗程,疗程间休息2d,共干预4个疗程。观察电针干预对大鼠体质量及下丘脑Dnmt1基因表达的影响。结果：造模后肥胖组和针刺组大鼠体质量均显著高于对照组（P〈0.05）;针刺组电针干预后体质量与针刺前相比明显下降（P〈0.05）,与对照组比较,差异无统计学意义（P〉0.05）。肥胖组大鼠下丘脑Dnmt1基因mRNA、蛋白质表达与针刺组、对照组比较,差异均无统计学意义（P〉0.05）。结论：电针可减轻食源性肥胖大鼠体质量,针刺治疗不改变该基因的活性。

英文摘要：

Objective：To observe the effect of electroacupuncture on the expression of DNA methyltransterase 1 （ Dnmtl ） in the hypothalamus of obese rats. Methods A total of 30 male Sprague - Dawley rats were randomized into control group（ n = 10）and high -fat group（ n = 20）. The rats in the high -fat group were given high -fat feed for 12 weeks to establish a rat model of diet - induced obesity. The model rats were divided into acupuncture group （ n = 7 ） and obese group （ n = 7 ）. After fixation, electroacupuncture was performed at Zhongwan, Tianshu, Sanyin- jiao, and Housanli for the rats in the acupuncture group. The intervention was performed for 4 courses;each course of treatment was 5 days,with 2 days for rest between each two courses. The effects of electroacupuncture on body weight and the expression of Dnmtl in the hypothalamus were observed. Results：After the model was established, the obese group and the acupuncture group had significantly higher body weight than the control group（ P 〈 0. 05 ） ; the acupuncture group had a significant decrease in body weight after electroacupuncture （ p 〈 0. 05 ）, with no signif- icant difference compared with the control group（ P 〉 0.05 ）. There were no significant differences in the mRNA and protein expression of Dnmtl in the hypothalamus between the obese group and the acupuncture and control groups （ P 〉 0. 05 ）. Conclusion ： Electroacupuncture can reduce the body weight of rats with diet - induced obesity, but it does not affect the activity of Dnmtl gene.