中文摘要：

背景与目的：研究Bid在维生素E琥珀酸酯（vitaminEsuccinate，VES）诱导人胃癌SGC-7901细胞凋亡中的作用。材料与方法：在VES诱导SGC-7901细胞凋亡过程中，检测Bid蛋白的活化；用RNA瞬时干扰阻断Bid的表达，再用VES作用SGC-790细胞后，比较Bid阴性表达和Bid阳性表达细胞的凋亡率、线粒体膜电位（AWm）、胞浆中细胞色素c含量和PARP活化程度。结果：VES作用细胞后Bid发生活化；Bid阴性表达细胞的凋亡率较Bid阳性表达细胞降低，线粒体膜电位升高，胞浆中细胞色素c含量减少，PARP活化减弱。结论：在VES诱导SGC-7901细胞凋亡过程中，Bid可能发挥重要作用。

英文摘要：

BACKGROUND AND AIM： To study the roles of Bid in vitamin E succinate （VES）-induced apoptosis of human gastric carcinoma SGC-7901 cells. MATERIALS AND METHODS： The activation of Bid was detected by western blot. Bid was blocked by transient RNA interference （RNAi）, and apoptotic rate, mitochondrial transmembrane potential （△Ψm）, the amount of cytosolic cytochrome C and the level of PARP cleavage were determined in Bid-negative cells and Bid-positive cells. RESULTS： Bid was activated by VES. Compared with Bid-positive cell, VES caused lower apoptotic rate, higher mitochondrial transmembrane potential, decreased release of cytochrome c to cytosol and lower level of PARP cleavage in Bid-negative cells. CONCLUSION： This study suggested Bid might play an important role in VES-induced apoptosis of human gastric carcinoma SGC-7901 cells.