中文摘要：

目的探讨精神应激（天敌应激）小鼠模型皮层、海马及下丘脑IL-1β，IL-6水平的变化，深入了解精神应激对脑组织中细胞因子的影响。方法随机将Balb／c小鼠分别暴露在正常环境（对照组）、急性（45min，1次）、慢性（45min／次，1次／d，连续14d）鼠类天敌（猫）应激环境中。应激结束后用EIA法检测血浆皮质酮水平，用ELISA法测定皮层、海马及下丘脑匀浆中IL-1β，IL-6的含量。结果①血浆皮质酮：应激后水平升高，以慢性应激组显著，与正常生理组比较差异有显著性（P〈0．05）；②皮层IL-1β，IL-6水平：急、慢性应激组水平均降低，以慢性应激组更显著，与正常对照及急性应激组差异均有显著性（P〈0．05）。③海马IL-1β，IL-6水平：急、慢性天敌应激后IL-1β水平降低，应激组与正常生理组比差异均具有显著性（P〈0．01）；IL-6水平无明显变化（P〉0．05）。④下丘脑IL-1β，IL-6水平：急性应激组IL-1β水平降低而慢性应激组水平略升高，但变化均差异无显著性（P〉0．05）；应激组IL-6水平均降低，以急性应激变化更为明显，其与正常对照组及慢性应激组相比差异有显著性（P〈0．05）。结论精神应激可影响小鼠脑组织中细胞因子的产生，细胞因子的变化在精神应激导致功能紊乱的过程中有重要作用。

英文摘要：

Objective To investigate IL-1β and IL- 6 levels in cortex, hippocampus and hypothalamus in a psychologically stressed mice model （cat -exposed predatory stress）, thus to further demonstrate the responses of neuro - immuno -encocrine network when subjected to psychological environments. Methods Mice were divided into 3 groups, normal control, acute and chronic stress group. Stressed mice, ie cat - exposed mice received either a single 45 - min cat exposure （ acute stress group） or daily 45 - min exposure to the cat for consecutive 14 days （ chronic stress group）. There- after, plasma corticosterone （CS） was detected by EIA and homogenization of cortex, hippocampus and hypothalamus was used for detection of IL -1β and IL - 6 by ELISA. Results （1）Plasma CS levels ： plasma CS was elevated in predatory stress mice, particularly in chronic stress group. There was a significant difference compared to normal control （P 〈 0.05）. （2）IL -1β and IL - 6 levels in cortex ： under predatory stress, both IL -1β and IL - 6 levels decreased and the chronic stress mice had the lowest level. A significant difference was revealed if compared with the normal and acute stress group （P 〈 0.05）. （3）IL -1β and IL - 6 levels in hippocampus ： IL -1β level decreased in both cat - exposed groups, and if compared with control group, there were significant differences between normal and stressed groups （P 〈 0.01 ）. IL -6 elevation in stressed group showed no significant differences compared to control group （P 〉 0.05 ）. （4） IL -1β and IL - 6 levels in hypothalamus ： for IL -1β, there was mild elevation in chronic stress group while decrease in acute group. Significant differences were not found between normal and stressed mice （ P 〉 0.05 ）. IL - 6 level exhibited decrease in both acute and chronic groups, with more prominent decreased in acute group. Furthermore, there were significant differ- ences if compared with normal group （P 〈 0.05）. Conclusion Psychologic