中文摘要：

目的：探讨结核性胸液中CD3＋ T、CD3＋ CD4＋ T、CD3＋ CD8＋ T细胞和CD4＋/CD8＋比值及细胞因子表达。方法：分离外周血单个核细胞和胸液细胞,流式细胞仪检测CD3＋、CD3＋ CD4＋、CD3＋ CD8＋和CD4/CD8比值。ELISA法检测胸液上清中IFN-γ、IL-2和TNF-α的水平。流式细胞仪检测CD3＋ CD4＋和CD3＋ CD8＋ T细胞Th1细胞因子IL-2、TNF-α、IFN-γ的产生。结果：与外周血相比,胸液细胞CD3＋ CD4＋ T和CD3＋ T细胞比例显著增加。ELISA结果表明,胸液上清中存在大量Th1型细胞因子。流式细胞仪分析结果表明,IL-2、TNF-α和IFN-γ主要由CD3＋ CD4＋ T细胞产生,而CD3＋CD8＋T细胞较少或不分泌细胞因子。结论：结核病人胸液中CD3＋和CD3＋ CD4＋ T细胞比例增加,胸液中存在大量Th1细胞因子,且主要来源于CD3＋ CD4＋ T细胞亚群,可能在抗结核免疫中发挥重要作用。

英文摘要：

Objective：To elucidate the phenotypic and functional characteristics of CD3＋ CD4＋ and CD3＋ CD8＋ T in pleural fluid of patients with tuberculous pleurisy.Methods：PBMCs and PFCs were isolated. The frequency of CD3＋,CD3＋ CD4＋,CD3＋ CD8＋ T cells and the ratio of CD4/CD8 in PFCs were analyzed by FACS. The concentration of IFN-γ,IL-2 and TNF-α in the pleural fluid were analyzed by ELISA.Production of IFN-γ,IL-2 and TNF-α by CD3＋ CD4＋ and CD3＋ CD8＋ T cells was detected by FACS following stimulation with BCG.Results：Higher frequencies of CD3＋ CD4＋ T cells and CD4＋ T/CD8＋ T ratio were demonstrated in PFCs when compared with PBMCs from normal donors and TB patients.ELISA analysis demonstrated that significantly high levels of IFN-γ and TNF-α were detected in pleural fluid.Further analysis by FACS indicated that IL-2,TNF-α and IFN-γ were predominantly secreted by CD3＋CD4＋T cells but not by CD3＋ CD8＋ T cells.Conclusion：The frequencies of CD3＋ and CD3＋ CD4＋ T cells are increased in PFCs. Large amounts of Th1 cytokines are detected,which are produced by CD3＋ CD4＋ T cells,and might play important roles against TB infection.