中文摘要：

以4-6月份采集感染柑橘衰退病毒（Citrus tristeza virus,CTV）样品L5的茎尖、叶柄和皮组织为材料,采用改进的病毒提纯方法获得了CTV的提纯病毒液。用提纯CTV免疫大耳白兔,所获抗血清的间接-ELISA效价为1：25600。利用制备的CTV多克隆抗体建立了A蛋白夹心（PAS-）ELISA、点免疫印迹（DIBA）和直接组织印迹（DTBIA）3种ELISA检测方法,并应用于湖北、湖南和江西3省主要柑橘产区CTV的检测,取得了理想的检测效果。

英文摘要：

Using the modified method citrus tristeza virus （ CTV ） was purified from stem tips, petioles and barks tissue of 15 collected from April to July. Polyclonal antibody was prepared by immunizing rabbits with purified particles of CTV and its titer was 1 ： 25600 in indirect ELISA. Protein A Sandwich （PAS） - ELISA, Dot - immunoblot assay （DIBA） and direct tissue blot immunoassay （DTBIA） were set up with the prepared polyelonal antibody. Then the antiserum and the established serological methods were used to detect CTV in the samples collected from Huhei, Hunan and Jiangxi provinces. The result showed that three serological methods all could be used to detect CTV in field samples quickly and efficiently.