中文摘要：

目的：制备具有pH响应的甲氧基聚乙二醇甲基丙烯酸-2-六亚甲基亚胺乙酯聚合物,测试材料pH功能响应,以及建立聚合物纳米粒载药方法.方法：通过核磁共振氢谱鉴定ATRP（Atom Transfer Radical Polymerization）聚合反应所获得的化合物结构.滴加-搅拌挥发法制备聚乙二醇甲基丙烯酸-2-六亚甲基亚胺乙酯纳米粒,酶标仪测定其载药量和包封率.透射电镜下观察其形态,激光粒度仪分析测定其粒径,包载DiR红外荧光探针检测纳米粒pH响应功能.结果：分别成功合成得到2-溴代异丁酸聚乙二醇单甲醚和甲基丙烯酸-2-六亚甲基亚胺乙酯单体.通过ATRP聚合反应成功合成聚乙二醇甲基丙烯酸-2-六亚甲基亚胺乙酯聚合物材料,并通过核磁氢谱对聚合材料进行鉴定.通过滴加搅拌法制备包载有模型药物香豆素-6的纳米粒,并对纳米粒的形态表征及载药量进行测定.结论：试验结果表明制备得到的聚合物纳米粒尺寸均匀,具有预期的pH响应效果,可以装载模型药物.

英文摘要：

Objective：Prepare pH sensitive PEG-C7AMA polymer material.Test polymer nanoparticle pH functional property and establish drug loading method.Methods：The 1HNMR is used to detect the polymer materials obtained from ATRP （Atom Transfer Radical Polymerization） polymerization reaction.Use Drop-stir evaporation method to prepare PEG-C7AMA nanoparticle and microplate reader to detect nanoparticle drug loading and entrapping efficiency.Use TEM （Transmission Electron Microscope）＆DLS （dynamic light scattering） to observe and analyze nanoparticle shape respectively.Polymer nanoparticle load DiR fluorescence probe is used to detect pH functional property with microplate reader.Results：Success to synthesis PEG macroinitiator and 2-azepane ethyl methacrylate respectively,which have been detected by 1HNMR spectrum.Success to synthesis PEG-C7AMA polymer material through ATRP polymerization reaction and 1HNMR to detect polymer materials.Through solvent-evaporation method to prepare nanoparticle which have been loaded coumarin-6,as a model drug,and to detect drug loading,entrapping efficiency and the shape of the established nanoparticle.Conclusion：Through experimental results show that the size of nanoparticle is on average.Nanoparticle have anticipated pH functional effect and can load model drug.