中文摘要：

目的：观察益气活血中药对脑出血大鼠脑组织中膜型基质金属蛋白酶、基质金属蛋白酶2和基质金属蛋白酶9表达量的影响,从脑出血损伤区微血管系统重建的角度,探讨益气活血中药治疗脑出血的作用机制。方法：实验于2006-03/10在中南大学湘雅医院中西医结合研究所实验室完成。实验材料：补阳还五汤全方（黄芪,当归,赤芍,红花,川芎,地龙,桃仁按20∶3∶3∶3∶2∶3∶3的比例）;补阳还五汤益气成分（黄芪按上述比例）;补阳还五汤活血成分（当归,赤芍,地龙,川芎,桃仁,红花按3∶3∶3∶2∶3∶3的比例）。用蒸馏水两次水煎,分别浓缩为1.54,0.81和0.73g/mL。实验分组：155只SD大鼠随机分为正常对照组、假手术组、模型组、益气活血组、益气组、活血组。正常对照组5只大鼠,其余每组30只,再随机分为术后灌胃2,4,7,14,21,28d6个观察时间点,各个时间点5只大鼠。实验干预：造模：采用立体定位技术将胶原酶Ⅶ注入大鼠大脑右苍白球制成脑出血大鼠模型。假手术组大鼠仅注入2μL生理盐水,其余手术过程相同。给药：正常对照组：普通饲养,自由饮水;假手术组和模型组术后予蒸馏水灌胃2次/d,2mL/次;益气活血组、益气组、活血组分别给予补阳还五汤全方、补阳还五汤益气成分、补阳还五汤活血成分30.80,16.20,14.60g/（kg·d）（按体表面积计算为临床70kg成人剂量的3倍）灌胃,2次/d,2mL/次。各组大鼠分别于灌胃2,4,7,14,21,28d麻醉下取脑,制备切片;正常组动物于28d处死。实验评估：免疫组织化学染色方法检测各组灌胃不同时间脑组织基质金属蛋白酶2、基质金属蛋白酶9和膜型基质金属蛋白酶的阳性微血管数。结果：155只大鼠均进入结果分析。①正常组、假手术组皮质偶见膜型基质金属蛋白酶、基质金属蛋白酶2和基质金属蛋白酶9表达。②模型组膜型基质金属蛋白酶、基质金?

英文摘要：

AIM： To investigate the action mechanism of yiqi huoxue （YQHX） therapy by observing the expressions of membrane type 1-matrix metalloproteinase （MT1-MMP）, MMP-2 And MMP-9 in intracerebral hemorrhagic （ICH） rat brains from the microvessel network reconstruction aspect. METHODS： The experiment was done in the laboratory of Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University from March to October in 2006. Experiment materials： Buyang Huanwu Decoction （milkvetch root, Chinese Angelica, red peony root, Carthamus tinctorius, Szechwan Lovage Rhizome, Pheretima asiatica, peach seed at the ratio of 20：3：3：3：2：3：3）, Yi-qi composition of Buyang Huanwu Decoction （milkvetch root at the same ratio）, Huo-xue composition of Buyang Huanwu Decoction （Chinese Angelica, red peony root, Carthamus tinctorius, Szechwan Lovage Rhizome, Pheretima asiatica, peach seed at the ratio of 3：3：3：2：3：3）. All the herbs were decocted twice with distilled water into 1.54,0.81 and 0.73 g/mE Experiment groups： 155 SD rats were randomly divided into six groups： normal control group （n =5）, sham group （n =30）, ICH group （n =30）, YQHX-treated group （n =30）, YQ-treated group （n =30）, HX-treated group （n =30）. Animals were observed at 2, 4, 7, 14, 21, 28 days postoperation, 5 rats at each time point. Experiment intervention： ICH model was induced by injecting collagenase type stereotaxically into right globus pallidus, while sham group was injected with 2 μL saline. Normal group was routinely fed. The sham group and ICH group were intragastricaUy administrated with distilled water, 2 μL once, twice daily. The animals of YQHX-treated group, YQ-treated group, and HX-treated group were respectively intragastrically administrated with Buyang Huanwu Decoction, the Yi-qi composition of Buyang Huanwu Decoction and the Huo-xue composition of Buyang Huanwu Decoction at the dosage of three times of the 70 kg adult according t