中文摘要：

目的：利用小干扰RNA（siRNA）沉默人结肠癌HCT-8细胞中雄激素受体（androgen receptor,AR）的表达,观察AR敲除对癌细胞增殖、迁移和侵袭能力的影响,初步探讨AR在结肠癌中的功能。方法：利用qRT-PCR和Western blot检测HCT-8细胞及正常人肠上皮细胞中AR的表达;siRNA转染HCT-8细胞沉默AR表达,利用qRT-PCR和Western blot检测转染效率;流式细胞术检测沉默AR后对HCT-8细胞周期的影响;CCK-8法检测沉默AR后对HCT-8细胞增殖的影响;Transwell实验检测沉默AR后对HCT-8细胞迁移及侵袭的影响。结果：AR在人结肠癌HCT-8细胞中高表达;siRNA能有效沉默HCT-8细胞中AR的mRNA及蛋白的表达。与对照组相比,沉默AR表达能够明显抑制HCT-8细胞的增殖、迁移和侵袭能力。结论：AR在人结肠癌HCT-8中高表达并能够促进癌细胞的增殖、迁移和侵袭能力。

英文摘要：

ObjectiveTo investigate the influence of androgen receptor （AR） down -regulation on cell prolifera-tion, migration and invasion of human colon cancer cells. Methods ： qRT - PCR and Western blot were used to detect the expression of AR in colon cancer cell HCT - 8 and human intestinal epithelial cell （ HIEC ）. Flow cytometry was used to detect the cell cycle. CCK - 8 assay was applied to test cell proliferation. Transwell assay was used to study cell migration and invasion. Results ： Compared with HIEC, AR expression in HCT - 8 cell was significantly higher. Compared with negative control group,the proliferation,migration and invasion ability were significantly decreased in HCT -8 cell when AR was silenced. Conclusion ： AR was highly expressed in HCT - 8 cell and could promote cell proliferation, migration and invasion.