中文摘要：

为分析不同禽源NDV毒株间囊膜蛋白抗原性的差异,作者以超滤纯化的鸡源NDV强毒WF00C株全病毒为免疫原制备NDV特异性单抗,并在其一般生物学特性研究和差异性比较的基础上,通过对其抗体可变区同源性比较及其基因家族中的归属性分析,进一步分析各株单抗的异同性;通过差异性作用位点区域氨基酸序列的分析与融合表达短肽反应性的验证,确定其差异表位关键性氨基酸位点。本研究中共鉴定筛选出了6株单抗：1B6、4B4、4E6、4E11、5H4和5H9,除4B4和5H4外,其余均具有血凝抑制（HI）活性和对WF00C株NDV的中和活性,但能够识别线性表位的1B6和4E11却对2株鸽源NDV无HI活性;单抗的异同性分析显示,1B6与4E11、4B4与5H4的抗体轻重链可变区基因家族来源均相同,1B6与4E11轻重链互补决定区氨基酸分别仅5个和1个氨基酸不同,而4B4与5H4则完全相同,4E6和5H9的基因来源和互补决定区氨基酸序列均差异较大;在NDV HN 14线性中和表位上的aa347位有6种氨基酸变化,鸽源毒株在此位点主要为甘氨酸（26/27）,而aa349位有3种变化,其中谷氨酸是鸽源毒株的特有指征;1B6和4E11所识别的差异性氨基酸E347和D349同为该表位关键性氨基酸。本研究所获得的3组生物学特性不同的6株抗NDV单抗,可归属为不同质的4种单抗;NDV HN aa349为E是鸽源毒株的特有指征,同时该位点也是线性中和表位上又一新的关键性氨基酸位点。

英文摘要：

To analyze antigenic diversity of envelope protein among NDV strains isolated from different avian,monoclonal antibodies（MAbs） against WF00C strains of NDV isolated from chicken were prepared by whole virus which was purified by ultrafiltration.Then,the homogeneity and heterogeneity of MAbs were further analyzed by homology of antibody variable region and ownership of gene family,and key amino acid sites on variable epitope were determined by amino acid sequence analysis of antigenic site and reaction with expressed peptide,which were on the basis of its general biological characteristics and diversity comparison.Six MAbs were identified in this study,and named as 1B6,4B4,4E6,4E11,5H4 and 5H9.Except 4B4 and 5H4,the rest MAbs had hemagglutination inhibition（HI） activity,and neutralizing activity to NDV WF00C strain.However,1B6 and 4E11 which recognized linear epitopes didn＇t react with two NDV strains isolated from pigeon in cross HI test.The homogeneity and heterogeneity analysis showed that antibody light and heavy chain variable region of 1B6 and 4E11,4B4 and 5H4 belonged to the same gene family.1B6 and 4E11 only had five and one amino acids difference in the light and heavy chain complementarity determining region,respectively,but 4B4 and 5H4 were identical.4E6 and 5H9 had obvious difference in gene sources and amino acid sequences of complementarity determining regions.Amino acid sequence analysis of NDV HN antigenic site 14 showed that site of aa347 had six kinds of amino acids,where glycine was common amino acid for this site of NDV strains isolated from pigeon（26/27）,and site of aa349 had three kinds of amino acids,where glutamate was specific to NDV strains isolated from pigeon.1B6 and 4E11 recognized amino acid E347 and D349 which were variable sites and crucial for these epitopes.In this study,six MAbs against NDV which belonged to three groups according to biological characteristics could be attributed to four types on the basis of homology.Site of aa349 on HN protein being glutamate was