中文摘要：

[目的]探讨ghrelin对蒙古绵羊脐静脉内皮细胞的血管内皮生长因子VEGF及其受体Fit-1mRNA表达的影响。[方法]试验分为4组：Ⅰ组，空白对照组；Ⅱ组，脂质体组（不加寡核苷酸）；Ⅲ组（SCON），20μmol／L正义寡核苷酸组；Ⅳ组（ASCON），20μmol／L反义寡核苷酸组。上述各组均在培养24、36、48h后，采用实时荧光定量方法检测VEGF及其受体Fit-1 mRNA表达情况的变化。[结果]VEGF和Fh-1熔解曲线表明扩增效果好，培养24h，VEGFmRNAⅠ组相对表达量为0．0769，Ⅱ组为0．0768，Ⅲ组为0．0769，3组之间无显著性差异（P〉0．05），Ⅳ组VEGFmRNA相对表达量下降为0．0242，且与Ⅰ、Ⅱ、Ⅲ组有显著性差异（P〈0．05）。培养36h，前3组相对表达量分别为0．059，0．051，0．0514，3组之间无显著性差异（P〉0．05），第Ⅳ组为0．0242，与前3组比较有显著性差异（P〈0．05）。培养48h，前3组相对表达量分别为0．061，0．0552，0．0521，3组之间无显著性差异（P〈0．05），第Ⅳ组为0．0115，与前3组比较有显著性差异（P〈0．05）。培养24h，Fit—lmRNA Ⅰ组相对表达量为0．0786；Ⅱ组为0．0782，Ⅲ组为0．0781，3组之间无显著性差异（P〉0．05），Ⅳ组Flt-1 mR—NA相对表达量下降为0．0256，且与Ⅰ、Ⅱ、Ⅲ组有显著性差异（P〈0．05）。培养36h，前3组相对表达量分别为0．0781，0．0784，0．0782，3组之间无显著性差异（P〉0．05），第Ⅳ组为0．0228，与前3组比较有显著性差异（P〈0．05）。培养48h，前3组相对表达量分别为0．0691，0．0780，0．0693，3组之间无显著性差异（P〉0．05），第Ⅳ组为0．0215，与前3组比较有显著性差异（P〈0．05）。可见，VEGF受体Flt-1 mRNA的表达与VEGF相似。二者均在Ⅰ组、Ⅱ组表达量无差异，具有较高的表达量，随着时间的延长没有明显变化；在Ⅲ组表达轻微下降，但与Ⅰ组和Ⅱ?

英文摘要：

[ Objective] This study was to investigate the effect of VEGF and its receptor Fit-1 mRNA expression in Mongolia sheep umbilical vein endothelial cells by ghrelin antisense inhibition. [ Method] Experiments were divided into 4 groups： group Ⅰ （blank control group） ; group Ⅱ （liposome group） ; group Ⅲ （SCON group： 20 μmol/L sense oligonucleotide） ; group Ⅳ （ASCON： 20 μmol/L antisense oligonucleotide）. VEGF and its receptor Fit-1 mRNA expression changes were detected by using real-time fluorescence quantitative detection after 24, 36 and 48 h. [ Result] The expression of VEGF mRNA in group Ⅰ, group Ⅱ were insignificantly different at higher expression levels, and did not change significantly with the time; the expression of VEGF mRNA in group Ⅲ assumed a slight decrease, but there were no significant differences between group I and group Ⅱ （P 〉0.05）, the expression of VEGF mRNA in group Ⅳ（antisense oligonucleotide group ） decreased significantly （P 〈 0.05） ; the expression of VEGF receptor FLT-1 mRNA was similar to that of VEGF. [ Conclusion] Antisense inhibition ghrelin has a downward effect to the expression of VEGF and its receptor Fit-1 the mRNA.