采用单胞藻(SA)、配合饵料(AF)、轮虫(BP)和卤虫幼体(BS)4种饵料,设计了SA+AF、SA+AF+BP、SA+AF+BS和SA+AF+BP+BS4种饵料组合并用于中国对虾苗种培育,投喂SA+AF饵料的受精卵分设“碘伏”消毒及未消毒组,投喂其他饵料的受精卵均为“碘伏”消毒组。分析各期幼体成活率、P10体重及WSSV携带量,结果表明,N—Z期间,受精卵消毒与否及投喂不同饵料对中国对虾的成活率差异均不显著(P〉0.05);Z—P期间,投喂BP的成活率明显高于未投喂BP组(P〈0.01);投喂SA+AF+BS的幼体在M—P期间成活率与其他饵料组差异极显著(P〈0.01);投喂SA+AF+BP饵料组P10幼体的平均体重小于投喂SA+AF+BP+BS组的幼体(P〈0.01);SA+AF消毒组P10仔虾WSSV携带量为10.52±3.3copies/ng DNA,低于其他饵料组仔虾的WSSV携带量(P〈0.05)。在P11-P60培育期间,分别投喂菲律宾蛤仔足肌(CF)+配饵(AF)、^60Coγ辐照菲律宾蛤仔足肌(RCF)+配饵(AF)、高锰酸钾消毒菲律宾蛤仔足肌(DCF)+配饵(AF)和配饵(AF)4种饵料组合。结果表明,CF+AF组对虾体重和体长增长最大,但与AF组差异不显著,而CF+AF组和AF组与其余两组差异显著(P〈0.05);4组对虾存活率差异不显著(P〉0.05)。WSSV人工感染实验结果表明,4种饵料投喂的对虾累积死亡率都在90%以上,差异均不显著(P〉0.05)。
Using microalgae (SA), artificial feed (AF), brachionus plicatilis (BP) and brine shrimp larvae (BS) , four diets including SA+AF, SA+AF+BP, SA+AF+BS, and SA+-AF+BP+BS were formulated and used for rearing Fenneropenaeus chinensis larvae. All fertilized eggs were disinfected except the larvae fed with SA+AF. The survival rates of differ- ent treatments were compared at each stage. It was found that there was no significant differ- ence in the survival rate at N-Z stage by feeding with different diets (P〉0.05), but the surviv- al rate of BP treatment was higher than others (P〈0.01) at Z-P stage, while that of SA+AF + BS treatment was significantly weight of 10-day post larvae (P10) different (P〈0.01) from fed with SA +AF+BP diet others at M-P stage. The body was less than that of SA+AF+ BP+BS (P〈0.01). The WSSV load of SA+AF treatment (10.52+3.3 copies/ng DNA) were significantly lower than that of other treatments (P〈0.05) at P10 stage. During the stage of P11-P60, four kinds of diets, including Ruditapes philippinarum foot muscle (CF)+artificial feed (AF), R. philippinarum foot muscle radiated by ^60Co γ (RCF)+artificial feed (AF), po- tassium-permanganate-disinfected R. philippinarum foot muscle (DCF)+artificial feed (AF) and artificial feed (AF), were used to feed the prawn. The CF+AF treatment gained the grea test increase of body weight and body length, showing no significant difference (P〉0.05) from AF treatment, but with significant difference (P〈0.05) from the other two treatments. The survival rate of CF+ AF treatment was the highest but no significant difference from other treatments (P〉0.05). WSSV artificial infection experiment showed that the accumulated mor- tality in all treatments were higher than 90 %, with no significant difference among treatments (P〉0.05).