中文摘要：

目的探讨B细胞异位基因2（B cell translocation gene 2,BTG2）在原发性肝细胞癌中的表达及与术后生存期之间的关系。方法共纳入125例可手术切除的原发性肝细胞癌患者,其中有完整随访记录101例;用免疫组化方法检测患者肿瘤组织中BTG2蛋白的表达,并分为3组：BTG2高表达组,BTG2蛋白中表达（＋＋）或强表达（＋＋＋）;BTG2低表达组,BTG2蛋白低表达（＋）;BTG2阴性组,BTG2蛋白无表达（-）。分析BTG2表达水平与临床病理因素和术后总生存期的相关性。选取12对癌与癌旁配对组织,用RT-PCR检测其BTG2表达水平。结果 125例癌组织中,BTG2阴性组、低表达组和高表达组分别为106、13、6例;癌旁组织中,BTG2阴性组、低表达组和高表达组分别为41、14、22例,癌与癌旁组织BTG2表达差异有统计学意义（χ~2=28.102,P〈0.001）。RT-PCR检测结果显示12对癌与癌旁配对组织中,癌组织BTG2表达水平低于癌旁的有8例。癌栓发生与BTG2表达有明显相关性（χ~2=8.305,P=0.013）,其中低表达组癌栓发生率最高。BTG2低表达组中位总生存期为36个月（95%CI：8.437~63.564）,而高表达和阴性表达组未达到中位总生存期（Log-Rankχ~2=4.512,P=0.105）。亚组分析表明,BTG2高表达组患者总生存期显著长于低表达组（χ~2=4.266,P=0.039）,而高表达与阴性组（χ~2=2.729,P=0.099）以及低表达与阴性组（χ~2=1.400,P=0.237）之间差异没有统计学意义。逐步Cox回归分析结果显示,分化程度、肝硬化、癌栓和转移情况是总生存期的独立预后因素。结论 BTG2蛋白在原发性肝细胞癌中的表达呈低表达,并且BTG2蛋白的高表达提示肝癌患者预后较好,可作为肝细胞癌的潜在预后指标。

英文摘要：

Objective To investigate the expression of B cell translocation gene 2（BTG2） in the patients with hepatocellular carcinoma（HCC）,and its relationship with postoperative prognosis. Methods One hundred and twenty-five patients with hepatocellular carcinoma confirmed pathologically and undergoing surgical treatment in our hospital from January 2012 to December 2014 were enrolled in this study. Among them,101 cases had complete follow-up data. According to the protein expression of BTG2 evaluated by immunohistochemical assay,the subjects were categorized to 3 groups,that is,high（＋ ＋/＋ ＋ ＋）,low（＋）and negative expression（-） groups. The correlation of BTG2 expression level with clinical pathological factors and overall survival were analyzed. Twelve pairs of tumor and para-carcinoma tissues were employed to detect the mRNA level of BTG2 by RT-PCR. Results In the obtained tumor tissues from 125 cases,there6,13 and 103 cases respectively with high,low and negative expression of BTG2,and the positive rates were22,14,41 respectively for the corresponding para-carcinoma tissues. The expression was significantly different between the tumor and para-carcinoma tissues（Chi square = 28. 102,P 0. 001）. The RT-PCR results showed that there were 8/12 pairs of tumor and para-carcinoma tissues with BTG2 expression level lower in the tumor tissue than the para-carcinoma tissue. Occurrence of tumor thrombus was significantly associated with BTG2 expression（Chi square = 8. 305,P = 0. 013）,and the low BTG2 expression group had the highest incidences of tumor thrombus. The median overall survival（m OS） was 36 months in the low BTG2 expression group（95% CI： 8. 436 ~ 63. 564）,while there was no m OS reached in the high expression and negative groups（Log-Rank Chi square = 4. 512,P = 0. 105）. However,the OS was significantly longer in the high than low expression groups（Chi square = 4. 266,P = 0. 039）. And there was no significant difference between the high and negative groups（Chi squ