中文摘要：

目的：探讨神经干细胞（NSCs）是否能分泌单胺类神经递质,且其所分泌的神经递质如5-羟色胺（5-HT）能否反馈调控NSCs的分化.方法：高效液相色谱检测分化7d的NSCs培养液与细胞裂解液中单胺类神经递质;应用免疫细胞化学及蛋白印迹,检测5-HT对NSCs分化以及细胞外信号调节激酶（ERK）、cAMP反应元件结合蛋白（CREB）表达的影响.结果：NSCs的培养液中富含肾上腺素（E）、去甲肾上腺素（NE）、5-HT、5-羟基吲哚乙酸（5-HIAA）和多巴胺的代谢产物高香草酸（HVA）,以5-HT与5-HIAA最多,且激动钠通道可以促进其分泌;细胞裂解液中的5-HT,是培养液中的20～24.6倍.5-HT能够上调pERK和pCREB的表达并促进NSCs的分化.结论：钠通道能促进NSCs分泌单胺类神经递质,其中的5-HT能够通过放大ERK、CREB信号转导途径,正反馈调节NSCs的进一步分化.

英文摘要：

Objective： To investigate whether the embryonic neural stem cells （NSCs） can secrete monoamine neurotran- smitters and whether the neurotransmitters can regulate the differentiation of NSC by feedback. Methods： Monoamine neurotransmitters in the culture medium and cell lysates were detected by high performance liquid chromatography （HPLC） in the NSCs （differentiated on day 7）. The impact of 5-TH on the differentiation of NSC and expressions of ERK and CREB were detected by immunocytochemistry and Western blotting. Results： Epinephrine, noradrenaline, 5-hydroxytryptamine（5- HT）, 5-HIAA（5-hydroxyindoleacetic acid） and homovanillic acid were rich in the culture medium of Nsc, in which 5-HT and 5-HIAA were the most abundant, and the activation of sodium channel couldt fhcilitate the paracrine/autocrine function of NSCs. 5-HT in the cell lysates is 20-24.6 times to controls; 5-HT up-regulated the expression of pERK and pCREB, and promoted the differentiation of NSCs. Conclusion： The 5-HT secreted by NSCs could positively improve the differentiation of NSCs by amplifying ERK and CREB signal transduction pathway.