中文摘要：

本实验旨在探究普安银鲫（Carassius auratus gibelio）卵黄囊仔鱼发育过程中乙酰辅酶A羧化酶（ACC）、脂肪酸合成酶（FAS）及肉毒碱棕榈酰转移酶Ⅰ（CPTⅠ）活性变化及葡萄糖和维生素C溶液分别浸泡对它们的影响。采用酶学方法测定分析了普安银鲫内源营养期、混合营养期和外源营养期ACC、FAS及CPTⅠ的活性变化特点。在卵黄囊仔鱼发育中,对照组与维生素C组中ACC和FAS活性呈上升趋势,CPTⅠ活性呈＂下降-上升＂变化趋势,而葡萄糖组ACC、FAS及CPTⅠ活性均呈上升趋势,且3种酶的活性均显著高于对照组（P〈0.05）。维生素C组ACC活性在内源营养期显著高于对照组,FAS活性在混合营养期和外源营养期显著高于对照组,CPTⅠ活性在内源营养期和外源营养期显著高于对照组（P〈0.05）。研究表明适宜水平的葡萄糖、维生素C能促进普安银鲫卵黄囊仔鱼发育中ACC、FAS和CPTⅠ的合成与分泌而形成新的代谢水平,以维持仔鱼体中脂质代谢的动态平衡。

英文摘要：

The experiment was conducted to study the effects of glucose and vitamin C on activities of acetylCo A carboxylase（ ACC）,fatty acid synthase（ FAS） and carnitine palmitoyltransferases Ⅰ（ CPT Ⅰ） during yolk-sac larvae development of Carassius auratus gibelio. Different concentrations of glucose and vitamin C were added to hatching water,respectively,until the yolk sac disappeared completely. The concentrations of glucose were 5 g / L,10 g / L,15 g / L,and 20 g / L,and the concentrations of vitamin C were 20 mg / L,25 mg / L,30 mg / L and 35 mg / L,respectively. The incubation time,hatching rate and survival rate of larvae were recorded during the process of experiment,and the activities of acetyl-Co A carboxylase,fatty acid synthase and carnitine palmitoyltransferases at endogenous nutrition stage,mix nutrition stage and exogenous nutrition stage of C. a. gibelio were analyzed by the method of enzymology,and data were analysed by one way ANOVA. Theresults showed that the activities of acetyl-Co A carboxylase and fatty acid synthase increased,while the acitvity of carnitine palmitoyltransferases showed＂down-up＂trend during yolk-sac larva development of C. a. gibelio in the control group and vitamin C group. While the activities of acetyl-Co A carboxylase,fatty acid synthase and carnitine palmitoyltransferases were all increased,and the activities of these three enzymes were significantly higher in the glucose group than those in the control group（ P 〈 0. 05）. The activity of acetyl-Co A carboxylase was significantly higher in experimental group than that in control group in endogenous nutrition stage,the activity of fatty acid synthase was significantly higher in mixed nutrition stage and exogenous nutrition stage,and the activity of carnitine palmitoyltransferases was significantly higher in endogenous nutrition stage and exogenous nutrition stage（ P 〈 0. 05）（ Fig. 1- 3）. The research showed that appropriate glucose concentration（ 15 g/L） and vitamin C concentration（ 30 mg/