中文摘要：

目的探讨创伤后精神应激障碍（PTSD）大鼠内侧前额皮质排斥性导向分子（RGMa）的表达变化。方法将45只健康成年雄性Wistar大鼠随机分为对照组（15只）、SPS7d组（15只）和SPSl4d组（15只），采用无连续单一应激（SPS）方法制备PTSD模型。用免疫组织化学和免疫印迹法检测内侧前额皮质RGMa的表达变化。结果免疫组织化学分析显示，对照组、SPS7d组和SPS14d组RGMa阳性信号OD值分别是（0．44±0．002）、（0．63±0．015）和（0．51±0．009），差异有统计学意义（P〈0．05）；免疫印迹分析显示，对照组、SPS7d组和SPSl4d组RGMa相对表达灰度值分别为（0．556817±0．00985）、（0．778967±0．028325）和（0．659633±0．030979），差异有统计学意义（P〈0．05）。结论PTSD大鼠内侧前额皮质RGMa表达增强，SPS7d达到高峰，RGMa的表达增加可能参与了PTSD的病理生理过程。

英文摘要：

Objective To observe the expression of repulsive guidance molecule A （RGMa） in the medial prefrontal cortex （mPFC） of rats with posttraumatic stress disorder（ PTSD ）. Methods Male Wistar rats were randomly divided into control group,SPS 7 d group and SPS 14 d group. The rats of SiX5 7 d and SPS 14 d groups were exposed to the single-prolonged stress （SPS） to set up the rat PTSD models. The expression of RGMa was detected using immunohistochemistry, Western blotting, image analysis and statistical analysis. Result The result of immunohistochemistry analysis showed that the expression level of RGMa gradually increased, peaking at SPS 7 d, and then gradually de- creased at SPS 14 d but still higher than in the control group （P 〈 0.05） . The Western blot result consistent with inmmnohistochemistry analysis. Conclusion The expression of RGMa was significantly increased in mPFC of PTSD model rats. The RGMa signal transduction pathway in mPFC might play an important role in the pathogenesis of PTSD.