中文摘要：

目的：研究2个不同年龄组供体来源的人视网膜微血管内皮细胞的特性。方法：分离培养2个不同年龄组的人视网膜微血管内皮细胞，分别为出生后30d内和40-65岁供体组。视网膜微血管内皮细胞具有特异性表达，通过免疫荧光染色CD31、vWF和乙酰化低密度脂蛋白（Dil—Ac—LDL）的摄取来定位内皮细胞的标记。通过使用matrigel基质胶培养细胞，激光共聚焦显微镜和Westernblotting分析vWF在细胞内的分布，评估2组细胞管状结构形成能力以及vwF在细胞内的不同分布。结果：从2个不同年龄组中成功地获得高纯度视网膜微血管内皮细胞。2组在种到基质胶后6h均能形成典型的二维管腔样结构，但婴幼儿组评分比成人组高（27．2％±2．2％），且二维管腔样结构在30h仍可较好地维持，而成人组已看到部分自发降解。另外可见到vWF在婴幼儿组主要表达在细胞核内，而成人组主要表达在胞浆。结论：不同年龄来源的人视网膜血管内皮细胞具有某些自身特定的性状特征，在与年龄相关的疾病的体外研究中，供体年龄因素可能造成的影响应该得到一定的重视。

英文摘要：

AIM： To investigate the properties of human retinal microvascular endothelial cells （RMECs） at two different age groups. METHODS： Human RMECs with high affinity were isolated from donors at two age groups： 30 d （group A） after birth and 40 -60 years of age （group B）. The RMECs were characterized for expression and localization of endothelial cell markers by immunofluorescence staining of CD31, yon Willebrand factor（vWF） and uptake of acetylated low -density lipoprotein. The ability of tube formation was assessed on Matrigel, and vWF distribution in ceils was ob- served by confocal immunofluorescence microscope and Western blotting analysis, respectively. RESULTS： High purity RMECs can be obtained readily from each group with modified methods. At 6 hours, cells from both groups formed tube structures successfully, but there was a significantly higher incidence of branching in RMECs of infant group （group A） by 27.2%± 2. 2% compared with adult group （group B） at 12 h （P 〈 0.05 ）. Group A maintained intact structure even at 30h, but group B partially lost the basic tube structure. In addition, vWF was translocated from cytoplasm to nucleus with aging. CONCLUSION： Human RMECs at different ages have specific properties. Cell properties related with age of the donors should be considered in in vitro studies.