目的:从细胞学水平上研究麦冬的菌根结构特征。方法:采用组织切片方法光学显微镜下观察麦冬的菌根形态和内部结构。结果:冬的根被细胞中存在大量真菌菌丝,菌丝能穿透根被细胞壁并在其中定殖,随后菌丝可进一步侵染外皮层细胞,受到真菌侵染的外皮层细胞壁不断加厚,加厚的细胞可达2~3层细胞;真菌能穿过这些厚壁细胞,进而侵入到皮层薄壁细胞。结论:层是真菌与植物相互作用的活跃部,但真菌并不能侵染麦冬根的髓部及中柱细胞。
OBJECTIVE To study the mycorrhizal structure and its characteristics of Ophiopogonjaponicus. METHODS Optical microscopy was used to observe the mycorrhizal characteristics of L. spicata and to reveal the relationship between endophytics and the host plant. RESULTS The results suggested that there were lots of hyphae beth inside and outside of the roots. The hyphae penetrated into velamina and propagate in the velamina cells. Then the hyphae invaded the exodermal cells whose cell wall was thickened gradual- ly,sometimes the exodermal cells was thickened by 2 or 3 layers. Although the exodermal cells wall became thicken, the hyphae still penetrated into the cortex. CONCLUSION The cortex is just the active region of interaction between mycorrhizal fungi and plant,and was the site of nutritional exchange. The fungi can't infect endodermis and pericycle of the roots.