用不同剂量(0~300 Gy)的60Co-γ射线辐照乌拉尔甘草种子,研究辐照处理对乌拉尔甘草种子在不同浓度NaCl(0~400 mmol/L)胁迫环境下萌发率和累积发芽率的影响,以期筛选出有助于提高乌拉尔甘草种子耐盐性能的辐射剂量.结果表明:随着NaCl浓度的增加,种子萌发率与NaCl浓度之间呈负相关.乌拉尔甘草种子萌发的“滞后”效应有加剧的趋势,主要表现为发芽率的降低、起始萌发时间的推迟以及达到最大发芽率所需时间的延长.但经100 Gy和300Gy的60Co-γ射线辐照处理的乌拉尔甘草种子,不仅提高了其在NaCl胁迫下的萌发率,而且缩短了种子的初始萌发时间,对NaCl胁迫表现出了较好的耐受性.
The study explored effect of 60Co-γ radiation on seeds germination and accumulated germination percentage of Glycyrrhiza uralensis Fisch under different concentration of NaCl stress,in order to select the best radiation dose which is helpful to improve salt-tolerance ability of Glycyrrhiza uralensis Fisch.The result showed a negative correlation between seeds germination percentage and salt concentration.Seed germination "lag" effect of Glycyrrhiza uralensis Fisch was shown and strengthen with salt concentration increasing.Salt stress not only reduced seeds germination percentage of Glycyrrhiza uralensis Fisch,but also postponed the initial seed germination time,and prolonged the seeds germination span.However,seeds of Glycyrrhiza uralensis Fisch exposed to the 100 Gy or 300 Gy radiation treatment showed higher tolerance to NaCl stress,the treatments not only significantly improved seeds germination percentage,but also shortened initial seed germination time.